The mechanism of the bidirectional regulation of the rat alpha-fetoprotein gene by glucocorticoid hormone.

Molecular and Cellular Endocrinology
J M DongJ F Chiu

Abstract

pAFP-CAT, a recombinant plasmid containing 5'-flanking sequence from -7 kb to +7 bp of rat alpha-fetoprotein (AFP) gene can drive the expression of the bacterial chloramphenicol acetyltransferase gene in McA-RH7777 and McA-RH8994 rat hepatoma cell lines. Dexamethasone treatment suppresses pAFP-CAT expression in McA-RH7777 cells but increases its expression in McA-RH8994 cells, which mimics the dexamethasone responses of the endogenous AFP gene in both cell lines. However, dexamethasone treatment enhanced pMMTV-CAT expression in both cell lines. These data suggest that the effects of dexamethasone on AFP gene expression may be mediated by different trans-acting factors binding to the specific cis-elements of the 5'-flanking region of the rat AFP gene.

References

Aug 28, 1979·Biochemical and Biophysical Research Communications·P CommerJ F Chiu
Jan 1, 1987·Journal of Steroid Biochemistry·M BeatoT Willmann
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D D MooreH M Goodman
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J Charron, J Drouin
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J T HoltA W Nienhuis
Jan 1, 1971·Advances in Cancer Research·G I Abelev
Jun 1, 1967·The Journal of Clinical Investigation·D Gitlin, M Boesman
Dec 21, 1981·Nucleic Acids Research·J F ChiuJ W Thanassi
Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard
Sep 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·William MartinDavid Penny

❮ Previous
Next ❯

Citations

Jul 1, 1994·Molecular and Cellular Endocrinology·Y LiuJ F Chiu
Jul 7, 2005·Liver International : Official Journal of the International Association for the Study of the Liver·Zobair M YounossiVikas Chandhoke
Jun 21, 1994·Biochimica Et Biophysica Acta·J P RabekJ Papaconstantinou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.