The mechanism of the resistance to stretch of isometrically contracting single muscle fibres.

The Journal of Physiology
L FusiGabriella Piazzesi

Abstract

Rapid attachment to actin of the detached motor domain of myosin dimers with one motor domain already attached has been hypothesized to explain the stretch-induced changes in X-ray interference and stiffness of active muscle. Here, using half-sarcomere mechanics in single frog muscle fibres (2.15 microm sarcomere length and 4 degrees C), we show that: (1) an increase in stiffness of the half-sarcomere under stretch is specific to isometric contraction and does not occur in rigor, indicating that the mechanism of stiffness increase is an increase in the number of attached motors; (2) 2 ms after 100 micros stretches (amplitude 2-8 nm per half-sarcomere) imposed during an isometric tetanus, the stiffness of the array of myosin motors in each half-sarcomere (e(m)) increases above the isometric value (e(m0)); (3) e(m) has a sigmoidal dependence on the distortion of the motor domains (Delta z) attached in isometric contraction, with a maximum approximately 2 e(m0) for a distortion of approximately 6 nm; e(m) is influenced by detachment of motors at z > 6 nm; (4) at the end of the 100 micros stretch the relation between e(m)/e(m0) and Delta z lies slightly but not significantly above that at 2 ms. These results support the idea that s...Continue Reading

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Citations

Jan 28, 2014·PloS One·Michael A FerencziAndrey K Tsaturyan
Dec 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Olena Pylypenko, Anne M Houdusse
May 30, 2015·International Journal of Molecular Sciences·Enrico Grazi
Dec 18, 2013·The Journal of Physiology·Luca FusiVincenzo Lombardi
Dec 18, 2013·The Journal of Physiology·Massimo ReconditiGabriella Piazzesi
Jul 13, 2014·The Journal of Physiology·Elisabetta BrunelloMassimo Reconditi
Feb 9, 2012·The Journal of Physiology·S Park-HolohanG Piazzesi
Jun 23, 2015·Biochemical and Biophysical Research Communications·Fábio C MinozzoDilson E Rassier
Dec 23, 2011·Muscle & Nerve·Emilie M SimoneauMarco V Narici
May 12, 2015·BioMed Research International·Alf MånssonGeorgios Tsiavaliaris
Aug 27, 2013·Biophysical Journal·Gerald Offer, K W Ranatunga
Nov 3, 2016·Biophysical Journal·Gerald Offer, K W Ranatunga
Dec 7, 2010·Journal of Muscle Research and Cell Motility·Gerald Offer, K W Ranatunga
Aug 20, 2010·American Journal of Physiology. Cell Physiology·Fabio C Minozzo, Dilson E Rassier
Jun 27, 2018·International Journal of Molecular Sciences·Alf MånssonDilson E Rassier
Jan 9, 2013·Journal of Muscle Research and Cell Motility·Marta NocellaBarbara Colombini

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