The mechanism of the tyrosine transporter TyrP supports a proton motive tyrosine decarboxylation pathway in Lactobacillus brevis

Journal of Bacteriology
Wout A M WolkenJuke S Lolkema

Abstract

The tyrosine decarboxylase operon of Lactobacillus brevis IOEB9809 contains, adjacent to the tyrosine decarboxylase gene, a gene for TyrP, a putative tyrosine transporter. The two genes potentially form a proton motive tyrosine decarboxylation pathway. The putative tyrosine transporter gene of L. brevis was expressed in Lactococcus lactis and functionally characterized using right-side-out membranes. The transporter very efficiently catalyzes homologous tyrosine-tyrosine exchange and heterologous exchange between tyrosine and its decarboxylation product tyramine. Tyrosine-tyramine exchange was shown to be electrogenic. In addition to the exchange mode, the transporter catalyzes tyrosine uniport but at a much lower rate. Analysis of the substrate specificity of the transporter by use of a set of 19 different tyrosine substrate analogues showed that the main interactions between the protein and the substrates involve the amino group and the phenyl ring with the para hydroxyl group. The carboxylate group that is removed in the decarboxylation reaction does not seem to contribute to the affinity of the protein for the substrates significantly. The properties of the TyrP protein are those typical for precursor-product exchangers tha...Continue Reading

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Citations

Mar 1, 2012·Critical Reviews in Food Science and Nutrition·Angela MarcobalRosario Muñoz
Jul 29, 2011·Critical Reviews in Food Science and Nutrition·Daniel M LinaresMaría Fernández
Jun 29, 2010·Applied and Environmental Microbiology·Emmanuel CotonJuke S Lolkema
Jan 15, 2013·Journal of Bacteriology·Andrea RomanoPatrick M Lucas
Mar 18, 2011·International Journal of Food Microbiology·Estefanía Muñoz-AtienzaCarmen Herranz
Mar 19, 2011·Microbiology·Monika CotonEmmanuel Coton
Apr 20, 2016·Frontiers in Microbiology·Pasquale RussoAntonio Bevilacqua
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Aug 30, 2016·Frontiers in Microbiology·Fausto GardiniFatih Özogul
Sep 1, 2018·FEMS Microbiology Letters·Rajni Hatti-KaulHesham El Enshasy
Mar 7, 2018·Scientific Reports·Beatriz Del RioMiguel A Alvarez
Dec 15, 2019·Cellular and Molecular Neurobiology·Katlynn Bugda GwiltGregory M Miller
Aug 10, 2021·Frontiers in Microbiology·Xiaoyu XuBin Wang

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