PMID: 40546Jul 15, 1979

The mechanism of transmembrane delta muH+ generation in mitochondria by cytochrome c oxidase

The Biochemical Journal
M LorussoS Papa


In rat liver mitochondria treated with rotenone, N-ethylmaleimide or oligomycin the expected alkalinization caused by proton consumption for aerobic oxidation of ferrocyanide was delayed with respect to ferrocyanide oxidation, unless carbonyl cyanide p-trifluoromethoxyphenylhydrazone was present. 2. When valinomycin or valinomycin plus antimycin were also present, ferricyanide, produced by oxidation of ferrocyanide, was re-reduced by hydrogenated endogenous reductants. Under these circumstances the expected net proton consumption caused by ferrocyanide oxidation was preceded by transient acidification. It is shown that re-reduction of formed ferricyanide and proton release derive from rotenone- and antimycin-resistant oxidation of endogenous reductants through the proton-translocating segments of the respiratory chain on the substrate side of cytochrome c. The number of protons released per electron flowing to ferricyanide varied, depending on the experimental conditions, from 3.6 to 1.5. 3. The antimycin-insensitive re-reduction of ferricyanide and proton release from mitochondria were strongly depressed by 2-n-heptyl-4-hydroxyquinoline N-oxide. This shows that the ferricyanide formed accepts electrons passing through the prot...Continue Reading


Nov 12, 1981·Biochimica Et Biophysica Acta·R P CaseyA Azzi
Feb 25, 1980·FEBS Letters·S PapaN Altamura
Jan 1, 1980·Annals of the New York Academy of Sciences·P Mitchell
Jan 1, 1981·European Journal of Biochemistry·S I TuJ F Marecek
Feb 1, 1983·Biological Reviews of the Cambridge Philosophical Society·W W Wainio
Apr 1, 1984·Journal of Bioenergetics and Biomembranes·J A Freedman, S H Chan
Dec 1, 1981·Journal of Bioenergetics and Biomembranes·R P CaseyA Azzi
Dec 1, 1994·Journal of Bioenergetics and Biomembranes·S PapaN Capitanio
Jul 1, 1981·European Journal of Biochemistry·F Di VirgilioG F Azzone

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Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
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