PMID: 3758939Sep 1, 1986Paper

The mechanisms of iron uptake by fetal rat hepatocytes in culture

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
D TrinderE Baker

Abstract

The mechanisms of iron accumulation by cultured hepatocytes isolated from fetal rat liver (19 days gestation) were investigated using rat transferrin labeled with 125I and 59Fe. The rates of iron and transferrin internalization by the cells were measured by incubating the hepatocytes with the labeled transferrin at 37 degrees C followed by treatment with pronase at 4 degrees C to remove surface-bound transferrin and iron. Iron internalization increased linearly with time. Approximately 65% of the internalized iron was incorporated into ferritin. In contrast to iron, the rate of transferrin internalization was biphasic, with a rapid phase during the first 10 to 15 min and a second slower phase which becomes more apparent after that time. Iron and transferrin internalization were temperature-dependent. Chase experiments showed that the internalized transferrin donated all of its iron to the cell and was then released in a biphasic manner which was dependent on the time of preincubation with radiolabeled transferrin. These experiments showed that iron uptake occurs by at least three processes. The first mechanism involves the specific receptor-mediated endocytosis of transferrin. Each cell has an average of 7.8 +/- 1.0 X 10(5) (me...Continue Reading

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Citations

Mar 28, 1989·Biochimica Et Biophysica Acta·K Osterloh, P Aisen
Jun 12, 1990·Biochimica Et Biophysica Acta·D R Richardson, E Baker
Jun 7, 1991·Biochimica Et Biophysica Acta·D Richardson, E Baker
Feb 19, 1991·Biochimica Et Biophysica Acta·D R Richardson, E Baker
Jan 1, 1991·Comparative Biochemistry and Physiology. A, Comparative Physiology·E H Morgan
Nov 21, 1998·Journal of Hepatology·R M GrahamE Baker
Mar 30, 2002·Critical Reviews in Oncology/hematology·Juliana C Kwok, Des R Richardson
Jun 7, 2002·Critical Reviews in Oncology/hematology·D R Richardson
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J Rogers, H Munro
Aug 5, 2010·The Journal of Biological Chemistry·Ningning ZhaoMitchell D Knutson
Mar 20, 2012·Antioxidants & Redox Signaling·Angelica M MerlotDes R Richardson
Jun 2, 2009·Cellular and Molecular Life Sciences : CMLS·Gregory Jon Anderson, Christopher D Vulpe
Feb 5, 2016·Biochimica Et Biophysica Acta·Danuta S KalinowskiDes R Richardson
Jan 5, 2001·Expert Opinion on Investigational Drugs·D R Richardson
Apr 9, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Anita C G ChuaDebbie Trinder
Dec 18, 2007·Journal of Hepatology·Ross M GrahamDebbie Trinder
Mar 20, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Chia-Yu Wang, Mitchell D Knutson
Mar 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E BakerP Ponka
Mar 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R SciotV J Desmet
Oct 16, 2009·American Journal of Physiology. Cell Physiology·Carly E HerbisonDebbie Trinder
Jan 1, 1988·Annals of the New York Academy of Sciences·E H Morgan, E Baker
Oct 19, 2007·Critical Reviews in Clinical Laboratory Sciences·Anita C G ChuaJohn K Olynyk
Jun 24, 2000·Endothelium : Journal of Endothelial Cell Research·R HallmannE Baker
Aug 4, 1998·The American Journal of Physiology·D Trinder, E Morgan

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