PMID: 2505680Oct 1, 1989Paper

The mechanisms of reduction of protein mixed disulfides (dethiolation) in cardiac tissue

Archives of Biochemistry and Biophysics
E M Park, J A Thomas

Abstract

Dethiolation of proteins (reduction of protein mixed disulfides) by NADPH-dependent and glutathione (GSH)-dependent enzymes, and by nonenzymatic reaction with GSH, was studied by electrofocusing methodology with glycogen phosphorylase b and creatine kinase as substrates. Phosphorylase b was not rapidly dethiolated by reduced glutathione alone, but a cardiac extract catalyzed rapid dethiolation by both an NADPH-dependent and a GSH-dependent process. In contrast, creatine kinase was actively dethiolated by GSH. This GSH-dependent dethiolation was not enhanced by a soluble extract of bovine heart. Creatine kinase was also not dethiolated by an NADPH-dependent process. Partial purification of the phosphorylase dethiolases showed that the NADPH-dependent dethiolase had both a high-molecular-weight and a low-molecular-weight component The properties of these components were similar to those of thioredoxin and thioredoxin reductase. These two components were sensitive to inhibition by phenylarsine oxide and inhibition was reversed by addition of a dithiol. In contrast, GSH-dependent dethiolation required a single component of low molecular weight. This process was less sensitive to phenylarsine oxide inhibition. These studies show tha...Continue Reading

References

Mar 1, 1985·Archives of Biochemistry and Biophysics·J F TurrensA L Lehninger
Apr 22, 1987·Biochimica Et Biophysica Acta·M W Collison, J A Thomas
Aug 15, 1986·Analytical Biochemistry·J A Thomas, D Beidler
Jan 1, 1985·Annual Review of Biochemistry·A Holmgren
Jul 1, 1972·The Biochemical Journal·A BoverisB Chance
Apr 1, 1966·Biochimica Et Biophysica Acta·P T Varandani, H H Tomizawa
Jun 1, 1984·Molecular and Cellular Biochemistry·S VijayaT Ramasarma
Jul 15, 1980·The Biochemical Journal·B Mannervik, K Axelsson

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Citations

Oct 18, 2003·Biochemical Pharmacology·Monica PallisNigel Russell
Aug 12, 2000·Free Radical Biology & Medicine·E A KonorevN Hogg
Jan 1, 1997·Free Radical Biology & Medicine·D W StarkeJ J Mieyal
May 3, 2002·European Journal of Biochemistry·Steven L ParkerMagnus M Berglund
Dec 19, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Carsten BerndtArne Holmgren

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