The mechanisms of reversible immobilization of fowl spermatozoa at body temperature

Journal of Reproduction and Fertility
K AshizawaK Okauchi

Abstract

Intact fowl spermatozoa became almost immotile at 40 degrees C, but motility increased significantly at 30 degrees C. The oxygen consumption at both temperatures was 8-11 microliters O2/10(10) spermatozoa.min-1. The ATP concentration at 40 degrees C was higher than that at 30 degrees C but ADP concentration at 30 degrees C was higher than that at 40 degrees C. Consequently, the ATP/ADP ratio at 30 degrees C (1.9-2.2) increased to 3.5-3.7 at 40 degrees C. The motility of intact spermatozoa at 40 degrees C was effectively restored by 2 mM-Ca2+, 10% seminal plasma and 10% peritoneal fluid taken at the time of ovulation. In contrast, these effectors did not restore the motility of demembranated spermatozoa at 40 degrees C. Motility of demembranated spermatozoa was restored at 30 degrees C. These results suggest that the immobilization of fowl spermatozoa at 40 degrees C occurs due to a decrease in flagellar dynein ATPase activity. Furthermore, the action of effectors for motility such as Ca2+ may not be directly on the axoneme, but mediated by solubilized substances which have been removed by demembranation of the spermatozoa.

Citations

Feb 11, 1999·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·K AshizawaY Tsuzuki
Jan 12, 1999·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·K AshizawaY Tsuzuki
Dec 8, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·K AshizawaY Tsuzuki
Jan 3, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·K AshizawaY Tsuzuki
Jun 1, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·K AshizawaY Tsuzuki
Jul 11, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·M LemoineE Blesbois
Jan 28, 1998·Molecular Reproduction and Development·K AshizawaY Tsuzuki
Oct 1, 1993·Molecular Reproduction and Development·A Nakanishi, A Iritani
May 3, 2005·Reproductive Medicine and Biology·Masaaki Morisawa, Manabu Yoshida
Jun 15, 1992·Biochemical and Biophysical Research Communications·K AshizawaY Tsuzuki

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