The Medicago truncatula Vacuolar iron Transporter-Like proteins VTL4 and VTL8 deliver iron to symbiotic bacteria at different stages of the infection process.

The New Phytologist
Jennifer H WaltonJanneke Balk

Abstract

The symbiotic relationship between legumes and rhizobium bacteria in root nodules has a high demand for iron, and questions remain regarding which transporters are involved. Here, we characterize two nodule-specific Vacuolar iron Transporter-Like (VTL) proteins in Medicago truncatula. Localization of fluorescent fusion proteins and mutant studies were carried out to correlate with existing RNA-seq data showing differential expression of VTL4 and VTL8 during early and late infection, respectively. The vtl4 insertion lines showed decreased nitrogen fixation capacity associated with more immature nodules and less elongated bacteroids. A mutant line lacking the tandemly-arranged VTL4-VTL8 genes, named 13U, was unable to develop functional nodules and failed to fix nitrogen, which was almost fully restored by expression of VTL8 alone. Using a newly developed lux reporter to monitor iron status of the bacteroids, a moderate decrease in luminescence signal was observed in vtl4 mutant nodules and a strong decrease in 13U nodules. Iron transport capability of VTL4 and VTL8 was shown by yeast complementation. These data indicate that VTL8, the closest homologue of SEN1 in Lotus japonicus, is the main route for delivering iron to symbioti...Continue Reading

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Citations

Aug 26, 2020·Journal of Experimental Botany·Rosario Castro-RodríguezManuel González-Guerrero
Dec 12, 2020·Computational and Structural Biotechnology Journal·Raquel Sorribes-DaudenSergi Puig
Dec 21, 2020·Physiologia Plantarum·Yuming SunShiwei Guo
Jan 8, 2021·International Journal of Molecular Sciences·David A Day, Penelope M C Smith
Mar 18, 2021·Journal of Experimental Botany·Janneke BalkSebastien Thomine

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Datasets Mentioned

BETA
DQ249171.1

Methods Mentioned

BETA
X‐ray
PCR
genotyping
confocal microscopy
chip
ICE
phosphotransferase
biosensor

Software Mentioned

ndi GO
Bio
imagej
Rad CFX Maestro

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