PMID: 9191099Jun 1, 1997Paper

The melanin-concentrating hormone gene in human: flanking region analysis, fine chromosome mapping, and tissue-specific expression

Brain Research. Molecular Brain Research
A VialeJ L Nahon

Abstract

Genomic sequences encoding the human melanin-concentrating hormone (MCH) were isolated from a YAC library and subcloned in pUC vector using a novel E. coli transformation method. A 4.1-kb fragment encompassing approximately 1.0 kb of the 5'-end-flanking region, the three exons-two introns of the coding region and approximately 1.7 kb of the 3'-end-flanking region, was sequenced. Comparison with the rat MCH gene indicated strong conservation in the 5'-flanking region, in particular over the putative TATA box, CAAT box, GRE and AP-1 elements that could potentially regulate MCH gene expression. FISH with a fluorescent MCH genomic probe on human chromosomes and PCR analysis of a YAC panel mapped MCH to chromosome 12q23.1 in a region flanked by D12S1074 and D12S1030 markers. Expression of the MCH RNA species and pro-MCH-derived peptides (MCH and NEI) was investigated in human tissues by combining Northern blotting, RT-PCR, in situ hybridization, immunohistochemistry and RIA. In the human brain, MCH mRNA and MCH/NEI peptides were predominantely expressed in the lateral hypothalamus in agreement with the known distribution of MCH expression in rat. In addition, MCH gene products were detected in extra-hypothalamic sites, such as the p...Continue Reading

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Citations

Oct 16, 2002·Biochemical and Biophysical Research Communications·Sophie E SchlumbergerAlex N Eberle
Sep 5, 1998·Brain Research·C L MillerR C Thompson
Jul 31, 2001·Molecular and Cellular Endocrinology·O VerlaetenA Bernard
Jul 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Efi KokkotouCharalabos Pothoulakis
Jun 18, 1998·Annals of the New York Academy of Sciences·A VialeJ L Nahon
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