The melanin-concentrating hormone receptor 1, a novel target of autoantibody responses in vitiligo

The Journal of Clinical Investigation
E Helen KempPhilip F Watson

Abstract

Vitiligo is a common depigmenting disorder resulting from the loss of melanocytes in the skin. The pathogenesis of the disease remains obscure, although autoimmune mechanisms are thought to be involved. Indeed, autoantibodies and autoreactive T lymphocytes that target melanocytes have been reported in some vitiligo patients. The objective of this study was to identify pigment cell antigens that are recognized by autoantibodies in vitiligo. Using IgG from vitiligo patients to screen a melanocyte cDNA phage-display library, we identified the melanin-concentrating hormone receptor 1 (MCHR1) as a novel autoantigen related to this disorder. Immunoreactivity against the receptor was demonstrated in vitiligo patient sera by using radiobinding assays. Among sera from healthy controls and from patients with autoimmune disease, none exhibited immunoreactivity to MCHR1, indicating a high disease specificity for Ab's against the receptor. Inhibition of MCH binding to its receptor by IgG from vitiligo patients was also shown.

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Citations

Aug 29, 2002·Clinical and Experimental Immunology·E A WatermanA P Weetman
Jul 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Efi KokkotouCharalabos Pothoulakis
Oct 20, 2009·The Journal of Clinical Endocrinology and Metabolism·E Helen KempAnthony P Weetman
May 20, 2009·Peptides·Bernard LakayeThierry Grisar
Feb 14, 2004·The Journal of Investigative Dermatology·Giovanni Di ZenzoGiovanna Zambruno
Jul 10, 2009·The Journal of Investigative Dermatology·Elizabeth A WatermanE Helen Kemp
Nov 25, 2003·The Journal of Investigative Dermatology·Raju V S R K GottumukkalaE Helen Kemp
Sep 7, 2011·Journal of Immunological Methods·Itziar López, Miguel Angel Pardo
Nov 30, 2011·Journal of the European Academy of Dermatology and Venereology : JEADV·V PradhanE H Kemp
Jul 31, 2007·Journal of the European Academy of Dermatology and Venereology : JEADV·N RezaeiE H Kemp
Jul 8, 2009·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·E Helen KempAnthony P Weetman
May 27, 2004·The Journal of Dermatology·Kamer GunduzFiliz Sebik
Mar 20, 2015·World Journal of Clinical Cases·Ghada F MohammedMohammed Saleh Al-Dhubaibi

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Methods Mentioned

BETA
phage-display
column chromatography
PCR
electrophoresis
cDNA
glycosylating

Software Mentioned

BLAST

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