The membrane anchor of mammalian brain acetylcholinesterase consists of a single glycosylated protein of 22 kDa

FEBS Letters
N Boschetti, U Brodbeck

Abstract

Mammalian brain acetylcholinesterase (AChE; EC 3.1.1.7) is membrane-bound through a structural subunit of about 20 kDa. So far little is known about this anchor because it is only detectable after hydrophobic labelling. In the present study we demonstrate that the two bands migrating around 20 kDa on SDS-PAGE derive from the same protein containing the same N-terminal amino acid sequence. The difference in their mobility is due to different N-glycosidation. Radioalkylation of cysteine residues reveals that the anchor contains just the two cysteine residues involved in binding the catalytic subunits.

References

Jan 1, 1977·Methods in Enzymology·U T Rüegg, J Rudinger
Mar 28, 1991·European Journal of Biochemistry·J SchallerE E Rickli
Oct 1, 1994·Journal of Neurochemistry·J LiaoU Brodbeck
Jan 1, 1994·Annual Review of Pharmacology and Toxicology·P Taylor, Z Radić
Jul 1, 1993·Progress in Neurobiology·J MassouliéF M Vallette
Sep 1, 1993·Journal of Neurochemistry·J LiaoU Brodbeck
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE

❮ Previous
Next ❯

Citations

Mar 16, 2013·Applied Biochemistry and Biotechnology·Habibe Budak KayaHasan Ozdemir
Apr 15, 2004·Neurochemistry International·Susana Nieto-CerónFrancisco J Campoy
Apr 18, 1997·Neuroscience Letters·M T Moral-NaranjoC J Vidal
Oct 28, 1998·Journal of Physiology, Paris·J MassouliéS Simon
Aug 2, 2003·Breast Cancer Research and Treatment·Francisco Ruiz-EspejoCecilio J Vidal
Jan 24, 2002·Neuron·Anselme L PerrierEric Krejci
Mar 12, 2013·International Journal of Biological Macromolecules·Bożena SosnowskaBożena Bukowska
Sep 15, 2012·Biological & Pharmaceutical Bulletin·Anna JarominArkadiusz Kozubek
Aug 24, 2000·The Journal of Biological Chemistry·A L PerrierE Krejci
Oct 15, 2009·Toxicology and Industrial Health·Sameeh A MansourTarek M Heikal

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.