The membrane form of the DNA repair protein Ku interacts at the cell surface with metalloproteinase 9

The EMBO Journal
Sylvie MonferranCatherine Muller

Abstract

The Ku heterodimer (Ku70/Ku80) plays a central role in DNA double-strand breaks repair. Ku is also expressed on the cell surface of different types of cells where its function remains poorly understood. From a yeast two-hybrid screen, we have identified a specific interaction between the core region of Ku80 and the hemopexin domain of metalloproteinase 9 (MMP-9), a key enzyme involved in the degradation of extracellular matrix (ECM) components. Ku associates with MMP-9 on the surface of leukemic cells as demonstrated by co-immunoprecipitation experiments in membrane extracts and double-label immunofluorescence studies. In normal and tumoral migratory cells, Ku80 and MMP-9 colocalize at the periphery of leading edge of cells and cellular invasion of collagen IV matrices was blocked by antibodies directed against Ku70 or Ku80 subunits as well as by Ku80-specific antisense oligonucleotides. Our results indicate that Ku and MMP-9 interact at the cell membrane of highly invasive hematopoietic cells of normal and tumoral origin and document the unexpected importance of the membrane-associated form of Ku in the regulation of ECM remodelling.

References

Jan 1, 1992·Autoimmunity·R G DalzielJ P Quinn
Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Oct 1, 1990·The Journal of Clinical Investigation·B S PrabhakarA L Notkins
Jan 1, 1995·Methods in Enzymology·G Murphy, T Crabbe
Mar 6, 1997·Biochemical and Biophysical Research Communications·P BelenguerB Ducommun
May 30, 1998·The Journal of Biological Chemistry·M W OlsonR Fridman
Jul 27, 1999·The Journal of Biological Chemistry·H Nagase, J F Woessner
Oct 12, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S M Ellerbroek, M S Stack
Feb 23, 2000·The Journal of Cell Biology·S S AkimovA M Belkin
Feb 15, 2001·Seminars in Cancer Biology·I Stamenkovic
Mar 14, 2001·American Journal of Physiology. Cell Physiology·E M LynchD V Faller
May 2, 2001·The Journal of Biological Chemistry·S M EllerbroekM S Stack
Sep 28, 2001·Trends in Immunology·G OpdenakkerJ Van Damme
Jan 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Gang LiEric A Hendrickson
May 23, 2002·Carcinogenesis·Stephen P Jackson
Mar 15, 2003·Nature Reviews. Cancer·John D Hood, David A Cheresh
Mar 26, 2003·Nature Cell Biology·Motoshi SawadaShigemi Matsuyama
Jul 8, 2003·The Journal of Pathology·Ivan Stamenkovic
Jul 25, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Thomas ZulianiMarie-Hélène Ratinaud
Mar 17, 2004·Journal of Molecular Biology·Sylvie MonferranBernard Salles

❮ Previous
Next ❯

Citations

Jun 26, 2013·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Josefa CabreroJuan Pedro M Camacho
Jan 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Paul J MintzRenata Pasqualini
Sep 15, 2010·The Journal of Biological Chemistry·Antoine DufourJian Cao
Jan 25, 2012·The Journal of Biological Chemistry·Maria QuanzMarie Dutreix
Aug 18, 2012·The Journal of Biological Chemistry·Jakub KryczkaCzesław S Cierniewski
Jul 29, 2009·Blood·Michael StefanidakisErkki Koivunen
Jan 17, 2013·Journal of Neuroinflammation·Delphine StephanSophie Desplat-Jégo
Jan 30, 2014·Cancers·Antonietta Rosella Farina, Andrew Reay Mackay
Dec 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Josée Guirouilh-BarbatBernard S Lopez
Apr 10, 2007·FEBS Letters·Alexander M BoutanaevDmitry I Nurminsky
Apr 17, 2012·Expert Opinion on Biological Therapy·Anna V CherepanovaPavel P Laktionov
Apr 4, 2013·Critical Reviews in Biochemistry and Molecular Biology·Jennifer VandoorenGhislain Opdenakker
Sep 4, 2010·Critical Reviews in Biochemistry and Molecular Biology·Bénédicte Cauwe, Ghislain Opdenakker
Mar 1, 2016·Oxidative Medicine and Cellular Longevity·Shihoko Kimura-Ohba, Yi Yang
Jan 19, 2016·Pathology, Research and Practice·Jun LiMeizuo Zhong
Dec 22, 2005·Experimental Cell Research·Vivian GamaShigemi Matsuyama
Jul 19, 2008·Journal of Cellular Physiology·Antoine DufourJian Cao
Aug 25, 2006·International Journal of Cancer. Journal International Du Cancer·Johan Fransson, Carl A K Borrebaeck
May 8, 2013·Biology of the Cell·Malgorzata ZimowskaMaria A Ciemerych
Nov 23, 2010·The FEBS Journal·Gillian Murphy, Hideaki Nagase
Jun 12, 2010·Annals of the New York Academy of Sciences·Czeslaw S CierniewskiRadoslaw Bednarek
Dec 8, 2010·Proteomics. Clinical Applications·Chann LagadecXuefen Le Bourhis
Sep 27, 2005·Experimental Cell Research·Jill Kuglin Schweitzer, Crislyn D'Souza-Schorey
May 24, 2005·Biochimica Et Biophysica Acta·Mikael Björklund, Erkki Koivunen
Mar 12, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Shuai WangZhenguo Sun
Apr 20, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Dermot O'SullivanAnnemarie Larkin
Feb 25, 2006·Cell·Javier Pizarro-Cerdá, Pascale Cossart
May 21, 2017·Biochimica Et Biophysica Acta. Molecular Cell Research·Parker G JobinChristopher M Overall
Oct 5, 2017·Nucleic Acids Research·Varun KumarPeter P Nawroth
Dec 23, 2006·Journal of Leukocyte Biology·Helene PiccardGhislain Opdenakker
Dec 7, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Josefa CabreroJuan Pedro M Camacho
Apr 26, 2008·Chembiochem : a European Journal of Chemical Biology·Dennis MerklePetra Schwille

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.