The membrane protein PrsS mimics σS in protecting Staphylococcus aureus against cell wall-targeting antibiotics and DNA-damaging agents

Microbiology
Christina N KruteLindsey N Shaw

Abstract

Staphylococcus aureus possesses a lone extracytoplasmic function (ECF) sigma factor, σ(S). In Bacillus subtilis, the ECF sigma factor, σ(W), is activated through a proteolytic cascade that begins with cleavage of the RsiW anti-sigma factor by a site-1 protease (S1P), PrsW. We have identified a PrsW homologue in S. aureus (termed PrsS) and explored its role in σ(S) regulation. Herein, we demonstrate that although a cognate σ(S) anti-sigma factor currently remains elusive, prsS phenocopies sigS in a wealth of regards. Specifically, prsS expression mimics the upregulation observed for sigS in response to DNA-damaging agents, cell wall-targeting antibiotics and during ex vivo growth in human serum and murine macrophages. prsS mutants also display the same sensitivities of sigS mutants to the DNA-damaging agents methyl methane sulfonate (MMS) and hydrogen peroxide, and the cell wall-targeting antibiotics ampicillin, bacitracin and penicillin-G. These phenotypes appear to be explained by alterations in abundance of proteins involved in drug resistance (Pbp2a, FemB, HmrA) and the response to DNA damage (BmrA, Hpt, Tag). Our findings seem to be mediated by putative proteolytic activity of PrsS, as site-directed mutagenesis of predicted...Continue Reading

References

May 1, 1972·Journal of General Microbiology·V SalisburyN Datta
Jun 15, 1980·Journal of Molecular Biology·P KarranE Seeberg
May 1, 1995·Molecular Microbiology·K L Brown, K T Hughes
Jun 1, 1993·Current Opinion in Cell Biology·D E BarnesB Sedgwick
Jan 1, 1997·Annual Review of Immunology·J MacMickingC Nathan
Feb 24, 2001·Nature·J C RainP Legrain
Aug 8, 2001·Molecular Microbiology·F CollandH De Reuse
Jan 30, 2002·FEMS Microbiology Letters·Robert E W Hancock, Annett Rozek
Jun 21, 2002·Advances in Microbial Physiology·John D Helmann
Feb 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Eyleen J O'RourkeJ Pablo Radicella
Mar 26, 2003·Molecular Plant-microbe Interactions : MPMI·R Nizan-KorenI Barash
Jul 2, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marcus S CookeJoseph Lunec
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Shunji NakanoPeter Zuber
Aug 12, 2004·Proceedings of the National Academy of Sciences of the United States of America·Taeok BaeDominique M Missiakas
Sep 21, 2004·Proteomics·Christine EymannMichael Hecker
Jan 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Monica L BurtsDominique M Missiakas
Jan 5, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·R Monina KlevensUNKNOWN National Nosocomial Infections Surveillance System
May 5, 2006·Journal of Bacteriology·Tomaz KoprivnjakJerrold P Weiss
Jun 22, 2006·Journal of Bacteriology·Sünje Johanna PampHanne Ingmer
Aug 18, 2006·The New England Journal of Medicine·Gregory J MoranUNKNOWN EMERGEncy ID Net Study Group
Oct 18, 2007·JAMA : the Journal of the American Medical Association·R Monina KlevensUNKNOWN Active Bacterial Core surveillance (ABCs) MRSA Investigators
Feb 1, 2011·Journal of Bacteriology·Mark AndersonDominique M Missiakas
Mar 16, 2011·Molecular Microbiology·Victoria R SteeleSimon J Foster
Apr 29, 2011·PloS One·Natalia MalachowaFrank R DeLeo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.