PMID: 6160473Aug 11, 1980Paper

The messenger RNA sequences in human fibroblast cells induced with poly rI.rC to produce interferon

Nucleic Acids Research
N B Raj, P M Pitha

Abstract

Induction of human fibroblast cells with poly rI.rC induces interferon mRNA which can be translated into interferon precursor in wheat germ cell free system or in Xenopus oocytes into biologically active interferon. The extent of gene expression in the poly rI.rC induced cells was compared to that of the uninduced cells by hybridization of the mRNA to complementary DNA. Homologous template driven hybridization of cDNA revealed the presence of two clearly defined transitions in the total poly A RNA from the induced cells; abundant class and a scarce class comprising approximately 37,000 diverse species of RNA. Heterologus hybridization of the cDNA with total uninduced mRNA showed that the majority of the mRNA sequences are the same in both the induced and uninduced cells. The results of the hybridization using cDNA prepared to the fraction enriched for interferon mRNA, however, showed that about 4% of the sequences present in the interferon enriched fraction are not present in the uninduced cells. These differences may result from the poly rI.rC induced alterations in gene expression.

References

Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D L Kacian, J C Myers
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M N ThangL Montagnier
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·N B Raj, P M Pitha
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R L CavalieriS Pestka
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M RubinsteinS Pestka
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C P LiuF H Ruddle
May 30, 1978·Biochemical and Biophysical Research Communications·B LebleuE De Clercq
Dec 11, 1975·Proceedings of the National Academy of Sciences of the United States of America·F H ReynoldsP M Pitha
Jul 19, 1974·Nature·J O BishopM Richardson

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Citations

Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·N B Raj, P M Pitha
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J ContentW Fiers
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·N B Raj, P M Pitha
Jan 1, 1985·Journal of Interferon Research·N B RajP M Pitha

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