The metabolic enzyme CTP synthase forms cytoskeletal filaments.

Nature Cell Biology
Michael Ingerson-MaharZemer Gitai

Abstract

Filament-forming cytoskeletal proteins are essential for the structure and organization of all cells. Bacterial homologues of the major eukaryotic cytoskeletal families have now been discovered, but studies suggest that yet more remain to be identified. We demonstrate that the metabolic enzyme CTP synthase (CtpS) forms filaments in Caulobacter crescentus. CtpS is bifunctional, as the filaments it forms regulate the curvature of C. crescentus cells independently of its catalytic function. The morphogenic role of CtpS requires its functional interaction with the intermediate filament, crescentin (CreS). Interestingly, the Escherichia coli CtpS homologue also forms filaments both in vivo and in vitro, suggesting that CtpS polymerization may be widely conserved. E. coli CtpS can replace the enzymatic and morphogenic functions of C. crescentus CtpS, indicating that C. crescentus has adapted a conserved filament-forming protein for a secondary role. These results implicate CtpS as a novel bifunctional member of the bacterial cytoskeleton and suggest that localization and polymerization may be important properties of metabolic enzymes.

References

Oct 1, 1977·Journal of Bacteriology·M Evinger, N Agabian
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·P BorkA Valencia
Jan 1, 1991·Methods in Enzymology·B Ely
Feb 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J S Zigler, P V Rao
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·X MaW Margolin
Jan 27, 1998·Journal of Structural Biology·D N Mastronarde
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·G KarimovaD Ladant
Jun 8, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·E A CarreyP F Watson
Dec 17, 2003·Cell·Nora AusmeesChristine Jacobs-Wagner
May 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Zemer GitaiLucy Shapiro
Jun 15, 2004·Journal of Structural Biology·Qingxiong S ZhengDavid A Agard
Sep 1, 2005·Journal of Structural Biology·Cristina V IancuGrant J Jensen
Sep 22, 2006·Molecular Microbiology·Ariane BriegelGrant J Jensen
Feb 6, 2007·Current Biology : CB·Ramanujam SrinivasanMohan K Balasubramanian
Apr 5, 2007·Nature Protocols·Cristina V IancuGrant J Jensen
Sep 15, 2007·Journal of Structural Biology·Fernando AmatMark Horowitz
Mar 28, 2008·Molecular Microbiology·Ariane BriegelGrant J Jensen
Jul 3, 2008·Genes & Development·Ramanujam SrinivasanMohan K Balasubramanian
Aug 30, 2008·Molecular and Cellular Biology·Stefan F PinterVirginia A Zakian
Sep 17, 2008·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·William F TivolGrant J Jensen
Mar 13, 2009·The EMBO Journal·Matthew T CabeenChristine Jacobs-Wagner
Apr 14, 2009·Journal of Structural Biology·Christian SulowayGrant J Jensen
May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·John N WernerZemer Gitai
May 7, 2009·Genes & Development·Godefroid CharbonChristine Jacobs-Wagner
May 12, 2009·Current Opinion in Microbiology·Zhuo Li, Grant J Jensen

❮ Previous
Next ❯

Citations

Nov 16, 2011·Quarterly Reviews of Biophysics·Lu Gan, Grant J Jensen
Aug 4, 2010·Nature Cell Biology·Matthew T Cabeen, Christine Jacobs-Wagner
Apr 12, 2013·Chemical Communications : Chem Comm·Hong ZhaoStephen J Benkovic
Dec 26, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jean-Philippe CastaingKumaran S Ramamurthi
Dec 12, 2012·Molecular & Cellular Proteomics : MCP·Malvina PapanastasiouAnastassios Economou
Nov 17, 2010·Molecular Biology of the Cell·Bonnie L Bassler
Jun 1, 2013·Molecular Biology of the Cell·James B Moseley
Nov 19, 2010·Cold Spring Harbor Perspectives in Biology·Clare L Kirkpatrick, Patrick H Viollier
Feb 19, 2013·Journal of Bacteriology·Katherine CellerGilles P van Wezel
Sep 9, 2011·Molecular Biology of the Cell·Ruth KabecheJames B Moseley
Feb 4, 2014·Molecular BioSystems·Jeremy D O'ConnellEdward M Marcotte
Jul 18, 2014·ELife·Rachael M BarryZemer Gitai
Nov 5, 2010·Annual Review of Genetics·Matthew T Cabeen, Christine Jacobs-Wagner
Jun 13, 2014·Molecular Biology of the Cell·Chalongrat NoreeJames E Wilhelm
Dec 3, 2014·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Gabriel N AugheyJi-Long Liu
Oct 13, 2012·Annual Review of Cell and Developmental Biology·Jeremy D O'ConnellEdward M Marcotte
Aug 7, 2013·The Journal of Cell Biology·Vladan LučičWolfgang Baumeister
Jan 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Chung Yu ChanStephen J Benkovic
Oct 22, 2011·Current Opinion in Microbiology·Rachael M Barry, Zemer Gitai
Oct 22, 2011·Trends in Cell Biology·Sunish Kumar Radhakrishnan, Patrick Viollier
Sep 21, 2011·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Kangni ChenJi-Long Liu
Mar 1, 2016·Nature Reviews. Microbiology·Catherine M OikonomouYi-Wei Chang
Jun 11, 2015·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Ömür Y Tastan, Ji-Long Liu
Mar 19, 2015·Current Biology : CB·Kimberly K Busiek, William Margolin
Jun 19, 2015·Journal of Structural Biology·H Jane DingGrant J Jensen
Sep 26, 2014·Metabolomics : Official Journal of the Metabolomic Society·B VaitheesvaranJ E Bruce
Jun 4, 2011·Molecular Nutrition & Food Research·José Antonio CurielFélix López de Felipe
Feb 27, 2013·The New Phytologist·Anna DoskočilováPavla Binarová
Mar 5, 2011·Molecular Microbiology·Johann Mignolet, Patrick H Viollier
Oct 18, 2011·Molecular Microbiology·Alex BöhmJörg Vogel
Jan 22, 2011·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Ji-Long Liu
Sep 12, 2015·Current Opinion in Microbiology·Kerwyn Casey Huang
Jun 18, 2016·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Qing-Ji ShenJi-Long Liu
Feb 3, 2016·The FEBS Journal·Kai SchuchmannVolker Müller
Nov 28, 2012·Current Opinion in Cell Biology·Martin Pilhofer, Grant J Jensen
Nov 19, 2011·FEMS Microbiology Reviews·Michael Ingerson-Mahar, Zemer Gitai

❮ Previous
Next ❯

Methods Mentioned

BETA
electron microscopy
fluorescence microscopy
light microscopy
two-hybrid

Software Mentioned

Tomo
Leginon
IMOD package
RAPTOR
UCSF
NIS Elements

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.