The metabolic pH response in Lactococcus lactis: an integrative experimental and modelling approach

Computational Biology and Chemistry
Ann Zahle AndersenLars Folke Olsen


Lactococcus lactis is characterised by its ability to convert sugar almost exclusively into lactic acid. This organic acid lowers extracellular pH, thus inhibiting growth of competing bacteria. Although L. lactis is able to survive at low pH, glycolysis is strongly affected at pH values below 5, showing reduced rate of glucose consumption. Therefore, in order to deepen our knowledge on central metabolism of L. lactis in natural or industrial environments, an existing full scale kinetic model of glucose metabolism was extended to simulate the impact of lowering extracellular pH in non-growing cells of L. lactis MG1363. Validation of the model was performed using (13)C NMR, (31)P NMR, and nicotinamide adenine dinucleotide hydride auto-fluorescence data of living cells metabolizing glucose at different pH values. The changes in the rate of glycolysis as well as in the dynamics of intracellular metabolites (NADH, nucleotide triphosphates and fructose-1,6-bisphosphate) observed during glucose pulse experiments were reproduced by model simulations. The model allowed investigation of key enzymes at sub-optimum extracellular pH, simulating their response to changing conditions in the complex network, as opposed to in vitro enzyme studi...Continue Reading


Jan 1, 1989·Critical Reviews in Microbiology·W N KoningsA J Driessen
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R OttoW N Konings
Sep 1, 1996·Current Microbiology·A HartkeY Auffray
May 3, 2000·International Journal of Food Microbiology·M MercadePascal Loubière
Aug 12, 2000·Journal of Bacteriology·B J KoebmannP R Jensen
Aug 31, 2000·Applied and Environmental Microbiology·N Asanuma, T Hino
Oct 17, 2002·Applied Microbiology and Biotechnology·Muriel Cocaign-BousquetPascal Loubière
Dec 13, 2003·Biochimica Et Biophysica Acta·Hiroshi Kobayashi
Mar 10, 2004·Applied and Environmental Microbiology·Ed W J van NielBärbel Hahn-Hägerdal
Apr 10, 2004·Bioinformatics·Brett G Olivier, Jacky L Snoep
May 12, 2004·Biochimica Et Biophysica Acta·Ming S LiuRichard J Sadus
Sep 6, 2005·Biochimica Et Biophysica Acta·Ming S LiuRichard J Sadus
Oct 26, 2005·Bio Systems·Jacky L SnoepHans V Westerhoff
Dec 31, 2005·Nucleic Acids Research·Robert D FinnAlex Bateman
Jul 18, 2006·Metabolic Engineering·Wietske A PoolOscar P Kuipers
Sep 20, 1943·The Journal of General Physiology·D E Goldman


Oct 25, 2011·Applied and Environmental Microbiology·Anisha GoelDouwe Molenaar
Oct 26, 2011·Microbial Cell Factories·Bas TeusinkDouwe Molenaar
Jan 30, 2013·Biophysical Chemistry·Jennifer LeveringSven Sahle
Dec 10, 2013·Nature Chemical Biology·Matthieu MasureelCédric Govaerts
Dec 4, 2012·Current Opinion in Biotechnology·Filipe Branco dos SantosBas Teusink
Sep 15, 2009·Current Opinion in Microbiology·Emily Kay, Brendan W Wren
Jun 29, 2011·The FEBS Journal·Katrin HübnerUrsula Kummer
Dec 3, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Cecilie K YttingLars Folke Olsen
May 5, 2010·Therapeutic Apheresis and Dialysis : Official Peer-reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy·Charles J Diskin
Mar 30, 2021·The Plant Journal : for Cell and Molecular Biology·Pascal HolzheuUrsula Kummer

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Glucose, (beta-D)-Isomer
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