The metabolic pH response in Lactococcus lactis: an integrative experimental and modelling approach

Computational Biology and Chemistry
Ann Zahle AndersenLars Folke Olsen

Abstract

Lactococcus lactis is characterised by its ability to convert sugar almost exclusively into lactic acid. This organic acid lowers extracellular pH, thus inhibiting growth of competing bacteria. Although L. lactis is able to survive at low pH, glycolysis is strongly affected at pH values below 5, showing reduced rate of glucose consumption. Therefore, in order to deepen our knowledge on central metabolism of L. lactis in natural or industrial environments, an existing full scale kinetic model of glucose metabolism was extended to simulate the impact of lowering extracellular pH in non-growing cells of L. lactis MG1363. Validation of the model was performed using (13)C NMR, (31)P NMR, and nicotinamide adenine dinucleotide hydride auto-fluorescence data of living cells metabolizing glucose at different pH values. The changes in the rate of glycolysis as well as in the dynamics of intracellular metabolites (NADH, nucleotide triphosphates and fructose-1,6-bisphosphate) observed during glucose pulse experiments were reproduced by model simulations. The model allowed investigation of key enzymes at sub-optimum extracellular pH, simulating their response to changing conditions in the complex network, as opposed to in vitro enzyme studi...Continue Reading

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Related Concepts

Glucose, (beta-D)-Isomer
Hydrogen-Ion Concentration
In Vivo NMR Spectroscopy
Lactococcus lactis subsp. lactis
Environment
Glucose
Glycolysis
Metabolism
Nicotinamide adenine dinucleotide (NAD)
NADH

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