The Metabolic Redox Regime of Pseudomonas putida Tunes Its Evolvability toward Novel Xenobiotic Substrates

MBio
Özlem AkkayaVíctor de Lorenzo

Abstract

During evolution of biodegradation pathways for xenobiotic compounds involving Rieske nonheme iron oxygenases, the transition toward novel substrates is frequently associated with faulty reactions. Such events release reactive oxygen species (ROS), which are endowed with high mutagenic potential. In this study, we evaluated how the operation of the background metabolic network by an environmental bacterium may either foster or curtail the still-evolving pathway for 2,4-dinitrotoluene (2,4-DNT) catabolism. To this end, the genetically tractable strain Pseudomonas putida EM173 was implanted with the whole genetic complement necessary for the complete biodegradation of 2,4-DNT (recruited from the environmental isolate Burkholderia sp. R34). By using reporter technology and direct measurements of ROS formation, we observed that the engineered P. putida strain experienced oxidative stress when catabolizing the nitroaromatic substrate. However, the formation of ROS was neither translated into significant activation of the SOS response to DNA damage nor did it result in a mutagenic regime (unlike what has been observed in Burkholderia sp. R34, the original host of the pathway). To inspect whether the tolerance of P. putida to oxidativ...Continue Reading

References

Nov 1, 1991·Applied and Environmental Microbiology·R J SpanggordK E Mortelmans
Jun 1, 1994·Journal of Bacteriology·B E HaiglerJ C Spain
Jan 1, 1995·Annual Review of Microbiology·J C Spain
Sep 18, 1997·Microbiology and Molecular Biology Reviews : MMBR·K Drlica, X Zhao
Dec 28, 1999·Methods : a Companion to Methods in Enzymology·W A Rosche, P L Foster
Mar 22, 2000·Chemical Research in Toxicology·J L BoltonT J Monks
May 2, 2000·Applied and Environmental Microbiology·S F NishinoJ C Spain
Jun 14, 2000·Current Opinion in Biotechnology·D T Gibson, R E Parales
Aug 23, 2001·Molecular Cell·H OhmoriR Woodgate
Jul 11, 2002·Journal of Bacteriology·Glenn R JohnsonJim C Spain
Aug 27, 2002·Annual Review of Pharmacology and Toxicology·Edward J Calabrese, Linda A Baldwin
Nov 26, 2003·Mutation Research·Jean CadetJean-Luc Ravanat
Jun 10, 2004·The Journal of Biological Chemistry·Lawrence P Wackett
Dec 18, 2004·Mutation Research·Patricia L Foster
Feb 5, 2005·Applied and Environmental Microbiology·Teca Calcagno Galvão, Víctor de Lorenzo
Sep 3, 2005·Nature Reviews. Microbiology·Christopher M Thomas, Kaare M Nielsen
Sep 20, 2005·Biochemical and Biophysical Research Communications·Daniel J FerraroS Ramaswamy
Oct 6, 2007·Critical Reviews in Biochemistry and Molecular Biology·Rodrigo S GalhardoSusan M Rosenberg
Oct 16, 2007·Journal of Bacteriology·Marc AbellaJordi Barbé
Oct 30, 2007·Journal of Bacteriology·Marianna A PatrauchanLindsay D Eltis
Dec 29, 2007·Ageing Research Reviews·Mark P Mattson
Mar 5, 2008·Cell Metabolism·David Gems, Linda Partridge
Mar 6, 2009·FEMS Microbiology Reviews·Alejandro Couce, Jesús Blázquez
Jul 22, 2009·Nature Chemical Biology·Shelley D Copley
May 29, 2010·Microbiology and Molecular Biology Reviews : MMBR·Kou-San Ju, Rebecca E Parales
Jan 25, 2011·Microbial Biotechnology·Robert A Kanaly, Shigeaki Harayama
Aug 2, 2011·Applied and Environmental Microbiology·Birgitta E EbertAndreas Schmid
Oct 4, 2011·Nature Reviews. Microbiology·Georg FuchsJohann Heider
Oct 27, 2011·Environmental Microbiology·Danilo Pérez-PantojaBernardo González

❮ Previous
Next ❯

Citations

Oct 27, 2018·Environmental Microbiology·Özlem AkkayaVíctor de Lorenzo
May 10, 2019·Biotechnology Journal·Lorena Fernández-CabezónPablo I Nikel
Jan 21, 2020·Journal of Medical Microbiology·Maia Kivisaar
Mar 24, 2020·Environmental Microbiology·A David Páez-EspinoVíctor de Lorenzo
Apr 11, 2020·Chembiochem : a European Journal of Chemical Biology·Manuel Nieto-Domínguez, Pablo I Nikel
Aug 14, 2020·Applied Microbiology and Biotechnology·Anna WeimerChristoph Wittmann
Jul 14, 2020·Critical Reviews in Biotechnology·Dominik Kopp, Anwar Sunna
Jan 1, 2020·Biotechnology for Biofuels·George L PeabodyAdam M Guss
Jan 5, 2021·Environmental Microbiology·Özlem AkkayaVíctor de Lorenzo
Feb 10, 2021·Environmental Microbiology·Alejandro Arce-RodríguezVictor de Lorenzo
Apr 13, 2021·ACS Synthetic Biology·Lorena Fernández-CabezónPablo I Nikel
Jul 10, 2021·Current Opinion in Biotechnology·Andrew J BorchertGregg T Beckham
Jul 6, 2021·Essays in Biochemistry·Nora Lisa BitzenhoferAnita Loeschcke
Sep 6, 2020·New Biotechnology·Víctor de LorenzoMarkus Schmidt

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
flow cytometry
biosensor
ICE

Software Mentioned

BitSize Bio
BioEdit

Related Concepts

Related Feeds

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.

Bioremediation (ASM)

Bioremediation is the treatment and removal of harmful pollutants or contaminants through the use of microorganisms. Discover the latest research here.

Bacterial Protein Structures (ASM)

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.