PMID: 43143Nov 15, 1979

The metabolism of 4-methyl-2-oxopentanoate in rat pancreatic islets

The Biochemical Journal
J C HuttonW J Malaisse

Abstract

1. Radioactively labelled 4-methyl-2-oxopentanoate was taken up by isolated pancreatic islets in a concentration- and pH-dependent manner and led to the intracellular accumulation of labelled amino acid and to a decrease in the intracellular pH. Uptake of 4-methyl-2-oxopentanoate did not appear to be either electrogenic or Na+-dependent. The islet content of 2-oxo acid radioactivity was not affected by either 2-cyano-3-hydroxy-cinnamate (10mM) or pyruvate (10mM), although both these substances inhibited the oxidation of [U-14C]4-methyl-2-oxopentanoate by islet tissue. 2. 4-Methyl-2-oxopentanoate markedly stimulated islet-cell respiration, ketone-body formation and biosynthetic activity. The metabolism of endogenous nutrients by islets appeared to be little affected by the compound. 3. Studies with the 3H- and 14C-labelled substrate revealed that 4-methyl-2-oxopentanoate was incorporated by islets into CO2, water, acetoacetate, L-leucine and to a lesser extent into islet protein and lipid. Carbon atoms C-2, C-3 and C-4 of the acetoacetate produced were derived from the carbon skeleton of the 4-methyl-2-oxopentanoate, but the acetoacetate carboxy group was derived from the incorporation of CO2. These results, and consideration of...Continue Reading

Citations

Jan 1, 1986·Diabetes/metabolism Reviews·M D Meglasson, F M Matschinsky
Aug 1, 1990·Biochemical Pharmacology·A Lynch, L Best
Feb 1, 1992·Bioscience Reports·L BestS Tomlinson
Jul 1, 1980·Naunyn-Schmiedeberg's Archives of Pharmacology·S KawazuW J Malaisse
Jul 1, 1983·Acta diabetologica latina·A SenerW J Malaisse
Jan 1, 1983·Annals of the New York Academy of Sciences·C W ParkinsJ Bancroft
Aug 22, 2015·The Journal of Biological Chemistry·Andrew David JamesJason Irving Edward Bruce
Aug 26, 2010·The Journal of Biological Chemistry·Yingsheng ZhouPengxiang She
Dec 25, 2013·International Journal of Molecular Medicine·W J Malaisse
Jan 22, 1998·The American Journal of Physiology·W J MalaisseA Sener
Jan 29, 2009·The Biochemical Journal·Javier Pizarro-DelgadoJorge Tamarit-Rodriguez

Related Concepts

Acetoacetates
Amination
Metazoa
Hexanoic Acids
Carbon Dioxide
Dinitrophenols
Hydrogen-Ion Concentration
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Lipids

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