The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes

Free Radical Biology & Medicine
Hua-Tao LiLin Feng

Abstract

The present study explored the apoptosis pathways in hydroxyl radicals ((∙)OH)-induced carp erythrocytes. Carp erythrocytes were treated with the caspase inhibitors in physiological carp saline (PCS) or Ca(2+)-free PCS in the presence of 40μM FeSO4/20μM H2O2. The results showed that the generation of reactive oxygen species (ROS), the release of cytochrome c and DNA fragmentation were caspase-dependent, and Ca(2+) was involved in calpain activation and phosphatidylserine (PS) exposure in (∙)OH-induced carp erythrocytes. Moreover, the results suggested that caspases were involved in PS exposure, and Ca(2+) was involved in DNA fragmentation in (∙)OH-induced fish erythrocytes. These results demonstrated that there might be two apoptosis pathways in fish erythrocytes, one is the caspase and cytochrome c-dependent apoptosis that is similar to that in mammal nucleated cells, the other is the Ca(2+)-involved apoptosis that was similar to that in mammal non-nucleated erythrocytes. So, fish erythrocytes may be used as a model for studying oxidative stress and apoptosis in mammal cells. Furthermore, the present study investigated the effects of glutamine (Gln)'s metabolites [alanine (Ala), citrulline (Cit), proline (Pro) and their combin...Continue Reading

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Citations

May 22, 2016·Apoptosis : an International Journal on Programmed Cell Death·Heejeong LeeDong Gun Lee
Feb 7, 2017·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Natalia I AgalakovaDina A Sufiyeva
Oct 27, 2018·Anais Da Academia Brasileira De Ciências·Jaqueline D Biller, Leonardo S Takahashi
Feb 21, 2021·Science Advances·Alexander Thiemicke, Gregor Neuert
May 22, 2018·Journal of Agricultural and Food Chemistry·Khaled OueslatiAlain Kondjoyan

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