The Methanosarcina acetivorans thioredoxin system activates DNA binding of the redox-sensitive transcriptional regulator MsvR

Journal of Industrial Microbiology & Biotechnology
Ryan SheehanDaniel J Lessner

Abstract

The production of biogas (methane) by an anaerobic digestion is an important facet to renewable energy, but is subject to instability due to the sensitivity of strictly anaerobic methanogenic archaea (methanogens) to environmental perturbations, such as oxygen. An understanding of the oxidant-sensing mechanisms used by methanogens may lead to the development of more oxidant tolerant (i.e., stable) methanogen strains. MsvR is a redox-sensitive transcriptional regulator that is found exclusively in methanogens. We show here that oxidation of MsvR from Methanosarcina acetivorans (MaMsvR) with hydrogen peroxide oxidizes cysteine thiols, which inactivates MaMsvR binding to its own promoter (P(msvR)). Incubation of oxidized MaMsvR with the M. acetivorans thioredoxin system (NADPH, MaTrxR, and MaTrx7) results in reduction of the cysteines back to thiols and activation of P msvR binding. These data confirm that cysteines are critical for the thiol-disulfide regulation of P(msvR) binding by MaMsvR and support a role for the M. acetivorans thioredoxin system in the in vivo activation of MaMsvR. The results support the feasibility of using MaMsvR and P(msvR), along with the Methanosarcina genetic system, to design methanogen strains with ...Continue Reading

References

Jan 1, 1983·Methods in Enzymology·P W RiddlesB Zerner
Sep 7, 2001·Annual Review of Microbiology·R C Fahey
Apr 5, 2002·Genome Research·James E GalaganBruce Birren
Jun 21, 2002·Advances in Microbial Physiology·James A Imlay
Jan 25, 2006·Molecular Microbiology·James A Imlay
Jul 1, 2008·Nature Reviews. Microbiology·Rudolf K ThauerReiner Hedderich
Dec 5, 2008·Archaea : an International Microbiological Journal·Adam M GussWilliam W Metcalf
Aug 21, 2009·Annual Review of Genetics·Yves MeyerJean-Philippe Reichheld
Mar 16, 2012·Bioresource Technology·Jo De VriezeWilly Verstraete
Jul 17, 2012·Journal of Bacteriology·James M Dubbs, Skorn Mongkolsuk
Jan 1, 1983·Systematic and Applied Microbiology·A Kiener, T Leisinger
Jul 19, 2013·The Journal of Biological Chemistry·Claudia M Cremers, Ursula Jakob
Feb 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Dwi SusantiBiswarup Mukhopadhyay
Aug 13, 2014·The FEBS Journal·Addison C McCarver, Daniel J Lessner

❮ Previous
Next ❯

Citations

Jan 27, 2019·Environmental Microbiology·Kerstin Fiege, Nicole Frankenberg-Dinkel
Oct 30, 2016·The Journal of Biological Chemistry·Dwi SusantiBiswarup Mukhopadhyay
May 10, 2020·Antioxidants·Mamta Rawat, Julie A Maupin-Furlow
Mar 16, 2019·Current Opinion in Biotechnology·Zhe Lyu, William B Whitman
Aug 16, 2021·The Science of the Total Environment·Anna PytlakZofia Stępniewska

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.