The methylene blue colorimetric microassay for determining cell line response to growth factors.

Cytotechnology
M F DentA P Wilson

Abstract

The validity of the methylene blue colorimetric microassay for determining the response of monolayers of human ovarian tumour cell lines to different growth factors was investigated. Linearity of the relationship between cell density and optical density was confirmed for each cell line (r=0.989-0.999,p<0.001), and when initial cell density was optimised to give exponential growth over the assay period, differences in response to medium supplements were obvious. The response of target cells to growth factors, obtained using the methylene blue assay, were compared with, and found to parallel, previously documented responses obtained non-colorimetrically. Thus Mink lung epithelial cells (MLEC) were inhibited by TGβ (Holleyet al., 1983), EGF had an inhibitory effect on A431 cells (Gill & Lazar, 1981; Barnes, 1982), and the mesothelial cell line showed a proliferative response to EGF and hydrocortisone (Connell and Rheinwald, 1983).The methylene blue colorimetric microssay was found to be a simple, reliable, sensitive method with low variability, for determining the response of cultured cells to growth factors.

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Citations

Jan 28, 2006·Assay and Drug Development Technologies·Hubert Th Wolterbeek, Astrid J G M van der Meer
Apr 18, 2012·Biochimica Et Biophysica Acta·Aliaksandr MikulichPetras Juzenas
Nov 2, 2016·Connective Tissue Research·Lucy E ClementsRoger K W Smith
Nov 1, 2018·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Ofer ShamniGalith Abourbeh
Jan 11, 2019·Bulletin of Experimental Biology and Medicine·K V RuttoE P Kiseleva
Aug 20, 2019·Journal of Microbiological Methods·Rebecca Jane WebbAlexandra A Roberts

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