The microbial diversity in picoplankton enrichment cultures: a molecular screening of marine isolates

FEMS Microbiology Ecology
Heike UphoffI Wagner-Döbler

Abstract

Picoplankton bacteria from a North Sea water sample were cultured under a variety of different conditions (nutrients, temperature, light, agitation, adhesion). Fluorescent in situ hybridization (FISH) analysis of the enrichments showed complex communities which were dominated by gamma-Proteobacteria or beta-Proteobacteria, followed by alpha-Proteobacteria and bacteria from the Cytophaga/Flavobacterium/Bacteroides (CFB) cluster. Among 410 isolates, a high degree of diversity was found, both with respect to colony color and morphology and with respect to genetic diversity. Isolated bacteria were classified into the main taxa by a special PCR approach, termed signature PCR (SIG-PCR). It was based on an oligo primer mixture targeting 16S rDNA which yielded PCR products of taxon-specific lengths. Again, gamma-Proteobacteria dominated (48%), followed by alpha-Proteobacteria (20%). beta-Proteobacteria were rarely isolated (eight strains of 410). The CFB cluster comprised the second largest phylum (14%), and 7.5% of all isolates belonged to the high-GC Gram-positives. Thus, isolated bacteria were representative of enrichment communities with the exception of the beta-Proteobacteria, which were detected in high abundance in certain enri...Continue Reading

References

May 1, 1992·Molecular Ecology·H HadrysB Schierwater
Jun 1, 1991·Applied and Environmental Microbiology·T B Britschgi, S J Giovannoni
Jan 1, 1991·Journal of Bacteriology·W G WeisburgD J Lane
Jul 1, 1991·Journal of Bacteriology·T M SchmidtN R Pace
Oct 1, 1990·International Journal of Systematic Bacteriology·H SchlesnerE Stackebrandt
May 3, 1990·Nature·S J GiovannoniK G Field
May 1, 1993·Applied and Environmental Microbiology·J A FuhrmanA A Davis
Oct 1, 1994·FEMS Microbiology Reviews·H HofstraJ van der Plas
Jul 15, 1994·FEMS Microbiology Letters·A E McCaigJ I Prosser
Jan 1, 1997·Applied and Environmental Microbiology·K G FieldS J Giovannoni
Mar 1, 1997·Applied and Environmental Microbiology·M T SuzukiS J Giovannoni
Aug 26, 1998·FEMS Microbiology Letters·S Y BandoC A Moreira-Filho
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·V V Yurkov, J T Beatty
Dec 3, 1998·Applied and Environmental Microbiology·K L Straub, B E Buchholz-Cleven
Jul 6, 2000·Applied and Environmental Microbiology·H EilersR Amann
Jul 6, 2000·Applied and Environmental Microbiology·I H BrümmerI Wagner-Döbler
Sep 30, 2000·Applied and Environmental Microbiology·J M GonzálezM A Moran
Oct 31, 2000·Applied and Environmental Microbiology·H EilersR Amann
Mar 1, 1993·Applied and Environmental Microbiology·D K ButtonB R Robertson
Sep 1, 1994·Microbial Ecology·J A FuhrmanR M Wilcox

❮ Previous
Next ❯

Citations

Oct 8, 2003·Journal of Microbiological Methods·Andreas D M FelskePaul de Vos
Jul 10, 2012·Trends in Biotechnology·Van H T Pham, Jaisoo Kim
Sep 1, 2006·Microbial Ecology·Ma Patricia V AzanzaCynthia T Hedreyda
Jan 30, 2020·Critical Reviews in Biotechnology·Paolo Stincone, Adriano Brandelli
Oct 6, 2005·Applied and Environmental Microbiology·Christopher B BlackwoodJeffrey S Buyer
Sep 6, 2003·Applied and Environmental Microbiology·Martin AllgaierIrene Wagner-Döbler
Jun 17, 2003·International Journal of Systematic and Evolutionary Microbiology·Irene Wagner-DöblerBrian J Tindall
Jun 15, 2007·Environmental Microbiology·Heike StevensMeinhard Simon
Jun 24, 2009·Extremophiles : Life Under Extreme Conditions·Karine Alain, Joël Querellou
Dec 13, 2019·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Natsuko Rivera-YoshidaMariana Benítez
May 11, 2005·Microbial Ecology·O G Brakstad, A G G Lødeng
Jan 21, 2009·Extremophiles : Life Under Extreme Conditions·Huirong LiBo Chen
Dec 8, 2005·FEMS Microbiology Ecology·Heike StevensThorsten Brinkhoff
Jun 5, 2012·Applied and Environmental Microbiology·Babur S Mirza, Jorge L M Rodrigues
Nov 26, 2002·Journal of Natural Products·Mohamed ShaabanHartmut Laatsch

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.