The microbial killing capacity of aqueous and gaseous ozone on different surfaces contaminated with dairy cattle manure

PloS One
Ameer MegahedJames Lowe

Abstract

A high reactivity and leaving no harmful residues make ozone an effective disinfectant for farm hygiene and biosecurity. Our objectives were therefore to (1) characterize the killing capacity of aqueous and gaseous ozone at different operational conditions on dairy cattle manure-based pathogens (MBP) contaminated different surfaces (plastic, metal, nylon, rubber, and wood); (2) determine the effect of microbial load on the killing capacity of aqueous ozone. In a crossover design, 14 strips of each material were randomly assigned into 3 groups, treatment (n = 6), positive-control (n = 6), and negative-control (n = 2). The strips were soaked in dairy cattle manure with an inoculum level of 107-108 for 60 minutes. The treatment strips were exposed to aqueous ozone of 2, 4, and 9 ppm and gaseous ozone of 1and 9 ppm for 2, 4, and 8 minutes exposure. 3M™ Petrifilm™ rapid aerobic count plate and plate reader were used for bacterial culture. On smooth surfaces, plastic and metal, aqueous ozone at 4 ppm reduced MBP to a safe level (≥5-log10) within 2 minutes (6.1 and 5.1-log10, respectively). However, gaseous ozone at 9 ppm for 4 minutes inactivated 3.3-log10 of MBP. Aqueous ozone of 9 ppm is sufficient to reduce MBP to a safe level, 6....Continue Reading

References

May 1, 1990·Applied and Environmental Microbiology·D G KorichC R Sterling
Sep 1, 1974·Mutation Research·C Hamelin, Y S Chung
Mar 1, 1982·Applied and Environmental Microbiology·T MasaokaT Kamiki
Nov 1, 1993·Journal of Dairy Science·A K GreeneJ C Serafini
Feb 5, 2000·The Journal of Hospital Infection·A D Russell
Feb 17, 2001·Trends in Biotechnology·P J Pomposiello, B Demple
Jun 1, 2002·Environmental Science & Technology·Glenn C Morrison, William W Nazaroff
Aug 22, 2002·Environmental Science & Technology·Tsung-Hung LiCharles J Weschler
Mar 30, 2006·Journal of the Air & Waste Management Association·Ahmet Aydogan, Mirat D Gurol
Oct 28, 2006·Journal of Food Protection·María Victoria SelmaMaría Isabel Gil
Oct 6, 2007·Journal of Applied Microbiology·L FanD Sharpe
May 19, 2009·Bioresource Technology·Dean O Cliver
Mar 11, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Ywu-Jang FuJuin-Yih Lai
May 1, 1942·The Journal of Hygiene·W J Elford, J van den Ende

❮ Previous
Next ❯

Citations

Sep 17, 2020·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·Reza Eftekhar AshtianiAmirali Zandinejad
Nov 24, 2020·Frontiers in Microbiology·Lucy ZhaoAlex V Chaves
Jan 21, 2021·Microorganisms·Angel Emilio Martínez de AlbaEnrique Monte
Feb 24, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Andréia Miho Morishita Harada, Maristela Silva Nascimento
Mar 6, 2021·Journal of Equine Veterinary Science·Jair Camargo FerreiraViviani Silva Rodrigues
Mar 12, 2021·European Journal of Dentistry·Magdalena Stawarz-JaneczekJolanta Pytko-Polończyk
Jun 3, 2021·Antibiotics·Angelica ArtasensiLaura Fumagalli
Jul 3, 2021·Biology·Laerte Marlon Conceição Dos SantosBruna Aparecida Souza Machado
Sep 1, 2020·ACS Nano·Nazmul KarimKostya S Novoselov

❮ Previous
Next ❯

Software Mentioned

FlexControl
SAS
MALDI Biotyper
Origin
Excel

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.