The Microbial Opsin Homolog Sop1 is involved in Sclerotinia sclerotiorum Development and Environmental Stress Response

Frontiers in Microbiology
Xueliang LyuJiasen Cheng

Abstract

Microbial opsins play a crucial role in responses to various environmental signals. Here, we report that the microbial opsin homolog gene sop1 from the necrotrophic phytopathogenic fungus Sclerotinia sclerotiorum was dramatically up-regulated during infection and sclerotial development compared with the vegetative growth stage. Further, study showed that sop1 was essential for growth, sclerotial development and full virulence of S. sclerotiorum. Sop1-silenced transformants were more sensitive to high salt stress, fungicides and high osmotic stress. However, they were more tolerant to oxidative stress compared with the wild-type strain, suggesting that sop1 is involved in different stress responses and fungicide resistance, which plays a role in the environmental adaptability of S. sclerotiorum. Furthermore, a Delta blast search showed that microbial opsins are absent from the genomes of animals and most higher plants, indicating that sop1 is a potential drug target for disease control of S. sclerotiorum.

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Citations

Jan 13, 2018·International Journal of Molecular Sciences·Alexander AdamUlrich Terpitz
Jun 30, 2018·Annual Review of Phytopathology·Liangsheng XuWeidong Chen
Jul 27, 2018·Phytopathology·Xiaofei Liang, Jeffrey A Rollins
Nov 29, 2016·Frontiers in Microbiology·Jie ZhongQian Zhou
Apr 27, 2019·Frontiers in Microbiology·Sabine PanzerUlrich Terpitz
Jul 8, 2020·International Journal of Molecular Sciences·Qing HeYuan Qin
Jan 13, 2021·Journal of Fungi·Silvia Rodríguez-PiresAntonieta De Cal
Mar 6, 2021·Journal of Agricultural and Food Chemistry·Li-Na DingXiao-Li Tan
Aug 4, 2021·Annual Review of Microbiology·Andrey RozenbergOded Béjà

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Datasets Mentioned

BETA
AAG01180
AAY82897
ACV05065
ABA08437
ABA08439
ABA08438
AAQ75384.1

Methods Mentioned

BETA
reverse transcription PCR

Software Mentioned

Phyre2
PhyML
BLAST
BLASTN
DELTA
Interactive Tree of Life
PyMOL
COBALT
ProtTest

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