PMID: 9174195Jun 1, 1997Paper

The microbicidal agent C31G inhibits Chlamydia trachomatis infectivity in vitro

Antimicrobial Agents and Chemotherapy
P B WyrickD Malamud

Abstract

Safe and effective vaginal microbicidal compounds are being sought to offer women an independent method for protection against transmission of sexually acquired pathogens. The purpose of this study was to examine the efficacy of two formulations of one such compound, C31G, against Chlamydia trachomatis serovar E alone, its host epithelial cell (HEC-1B) alone, and against chlamydiae-infected HEC-1B cells. Preexposure of isolated, purified infectious chlamydial elementary bodies (EB) to C31G, at pHs 7.2 and 5.7, for 1 h at 4 degrees C resulted in reduced infectivity of EB for HEC-1B cells. Examination of the C31G-exposed 35S-EB on sodium dodecyl sulfate-polyacrylamide gel electrophoresis autoradiographs and by Western blotting revealed a C31G concentration-dependent and pH-dependent destabilization of the chlamydial envelope, resulting in the release of chlamydial lipopolysaccharide and proteins. Interestingly, when the host human genital columnar epithelial cells were infected with chlamydiae and then exposed to dilute concentrations of C31G which did not alter epithelial cell viability, chlamydial infectivity was also markedly reduced. C31G gained access to the developing chlamydial inclusion causing damage to or destruction of...Continue Reading

References

Feb 1, 1991·The Journal of Infectious Diseases·F A PlummerA R Ronald
Aug 1, 1989·Infection and Immunity·P B WyrickC R Bagnell
Jul 1, 1987·Sexually Transmitted Diseases·S T KnightP B Wyrick
Oct 1, 1988·American Journal of Obstetrics and Gynecology·A S MaslowP B Wyrick
Mar 1, 1988·Antimicrobial Agents and Chemotherapy·A M CornerD Malamud
Oct 1, 1985·The Journal of Infectious Diseases·J FribergE Confino
Jan 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·B Rotman, B W Papermaster
Jul 1, 1984·Fertility and Sterility·P Wølner-Hanssen, P A Mårdh
Jun 1, 1994·American Journal of Public Health·M Potts
Mar 1, 1996·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·R Pauwels, E De Clercq

❮ Previous
Next ❯

Citations

Aug 26, 1998·Sexually Transmitted Diseases·S L RosenthalL R Stanberry
May 27, 2005·Antimicrobial Agents and Chemotherapy·Tsutomu YamazakiYukihiko Hara
Mar 18, 2011·Reviews in Medical Virology·Kevin K AriënGuido Vanham
Jun 13, 2001·Infection and Immunity·A Y JongK S Kim
Aug 19, 2014·Journal of Oleo Science·Mohamed F Zaki, Salah M Tawfik
Jul 4, 1998·Biochimica Et Biophysica Acta·C W McConlogueT K Vanderlick
Sep 13, 2005·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Bradley J CataloneBrian Wigdahl
Mar 6, 2007·Lancet·Jan Balzarini, Lut Van Damme

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR Screens in Drug Resistance

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of CRISPR-Cas system in high-throughput genome-wide screens to identify genes that may confer drug resistance.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.