A clinical study was undertaken to investigate and compare specifically the aerobic and anaerobic microbiology of infected and noninfected leg ulcers. Leg ulcers, defined as being infected on the basis of clinical signs, were swab sampled and investigated for aerobic and anaerobic microorganisms using stringent isolation and identification techniques. Two hundred and twenty isolates were cultured from 44 infected leg ulcers, in comparison with 110 isolates from 30 noninfected leg ulcers. Statistical analysis indicated a significantly greater mean number of anaerobic bacteria per infected ulcer (particularly Peptostreptococcus spp. and Prevotella spp.) in comparison with the noninfected ulcer group (2.5 vs. 1.3, respectively) (P < 0.05). Also, anaerobes represented 49% of the total microbial composition in infected leg ulcers compared with 36% in noninfected leg ulcers. The mean numbers of aerobes per wound in the two ulcer groups were not statistically different (P > 0.05). The study failed to demonstrate a clear correlation between commonly implicated facultative pathogens and wound infection. The isolation rate of Pseudomonas aeruginosa was generally low and, although Staphylococcus aureus was a frequent isolate in both wound...Continue Reading
Enhancement of growth of aerobic, anaerobic, and facultative bacteria in mixed infections with anaerobic and facultative gram-positive cocci
Successful prophylaxis with tinidazole of infection after major head and neck surgery for malignant disease
Use of an anaerobic collection and transport swab device to recover anaerobic bacteria from infected foot ulcers in diabetics
Panbacterial real-time PCR to evaluate bacterial burden in chronic wounds treated with Cutimed™ Sorbact™
Specific protease activity indicates the degree of Pseudomonas aeruginosa infection in chronic infected wounds
Microbiology of chronic leg and pressure ulcers: clinical significance and implications for treatment
Deep tissue biopsy vs. superficial swab culture monitoring in the microbiological assessment of limb-threatening diabetic foot infection
An in vitro model of chronic wound biofilms to test wound dressings and assess antimicrobial susceptibilities
Bacteriology of moderate-to-severe diabetic foot infections and in vitro activity of antimicrobial agents
Survey of bacterial diversity in chronic wounds using pyrosequencing, DGGE, and full ribosome shotgun sequencing
Clinical identification of bacteria in human chronic wound infections: culturing vs. 16S ribosomal DNA sequencing
Polymicrobial nature of chronic diabetic foot ulcer biofilm infections determined using bacterial tag encoded FLX amplicon pyrosequencing (bTEFAP)
Success rate of split-thickness skin grafting of chronic venous leg ulcers depends on the presence of Pseudomonas aeruginosa: a retrospective study
The Wound Microbiome: Modern Approaches to Examining the Role of Microorganisms in Impaired Chronic Wound Healing
Changes in inflammatory gene expression induced by hyperbaric oxygen treatment in human endothelial cells under chronic wound conditions
Non-healing is associated with persistent stimulation of the innate immune response in chronic venous leg ulcers
In vitro susceptibility of methicillin-resistant and methicillin-susceptible strains of Staphylococcus aureus to two different cold atmospheric plasma sources
Treating the chronic wound: A practical approach to the care of nonhealing wounds and wound care dressings
Anaerobic cocci populating the deep tissues of chronic wounds impair cellular wound healing responses in vitro
Manuka honey vs. hydrogel--a prospective, open label, multicentre, randomised controlled trial to compare desloughing efficacy and healing outcomes in venous ulcers
Laser irradiation effect on Staphylococcus aureus and Pseudomonas aeruginosa biofilms isolated from venous leg ulcer
Proteome analysis reveals antiangiogenic environments in chronic wounds of diabetes mellitus type 2 patients
The clinical efficacy of two semi-quantitative wound-swabbing techniques in identifying the causative organism(s) in infected cutaneous wounds
Novel noninvasive identification of biomarkers by analytical profiling of chronic wounds using volatile organic compounds
The effect of negative pressure wound therapy with periodic instillation using antimicrobial solutions on Pseudomonas aeruginosa biofilm on porcine skin explants
A prospective, open, multicentre study to evaluate a new gelling fibre dressing containing silver in the management of venous leg ulcers
Bacterial isolates from infected wounds and their antibiotic susceptibility pattern: some remarks about wound infection
Skin and wound decontamination of multidrug-resistant bacteria by cold atmospheric plasma coagulation
Safety and performance evaluation of a next-generation antimicrobial dressing in patients with chronic venous leg ulcers
Which medical device and/or which local treatment are to be used, as of 2012, in patients with infected pressure sore? Developing French guidelines for clinical practice
Real-time PCR assays compared to culture-based approaches for identification of aerobic bacteria in chronic wounds
Does antibiotic resistance impair plasma susceptibility of multi-drug resistant clinical isolates of enterococci in vitro?
Virulence determinants in clinical Staphylococcus aureus from monomicrobial and polymicrobial infections of diabetic foot ulcers
Pseudomonas aeruginosa infection alters the macrophage phenotype switching process during wound healing in diabetic mice
Inter-rater and intra-rater reliability outcomes of a rapid bacteria counting system with pressure ulcer samples
In vitro studies on medicinal plants used against bacterial diabetic foot ulcer (BDFU) and urinary tract infected (UTI) causing pathogens
A multimodel regime for evaluating effectiveness of antimicrobial wound care products in microbial biofilms
Role of the wet-to-dry phase of cleansing in preparing the chronic wound bed for dressing application
Culture-Based Screening of Aerobic Microbiome in Diabetic Foot Subjects and Developing Non-healing Ulcers
T4 lysozyme fused with cellulose binding module for antimicrobial cellulosic wound dressing materials
Does noncontact low-frequency ultrasound therapy contribute to wound healing at the molecular level?
Impaired host response and the presence of Acinetobacter baumannii in the serum microbiome of type-II diabetic patients
Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.
Chronic Fatigue Syndrome
Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.
Systemic Juvenile Idiopathic Arthritis
Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.
Chromatin Regulation and Circadian Clocks
The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.
Central Pontine Myelinolysis
Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.
Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.
Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.
Cell Atlas Along the Gut-Brain Axis
Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.
Chronic Traumatic Encephalopathy
Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.