The microbiome modulating activity of bile acids.

Gut Microbes
Yuan TianAndrew D Patterson

Abstract

Bile acids are potent antibacterial compounds and play an important role in shaping the microbial ecology of the gut. Here, we combined flow cytometry, growth rate measurements (OD600), and NMR- and mass spectrometry-based metabolomics to systematically profile the impact of bile acids on the microbiome using in vitro and in vivo models. This study confirmed that (1) unconjugated bile acids possess more potent antibacterial activity than conjugated bile acids; (2) Gram-positive bacteria are more sensitive to bile acids than Gram-negative bacteria; (3) some probiotic bacteria such as Lactobacillus and Bifidobacterium and 7α-dehydroxylating bacteria such as Clostridium scindens show bile acid resistance that is associated with activation of glycolysis. Moreover, we demonstrated that (4) as one of most hydrophobic bile acids, lithocholic acid (LCA) shows reduced toxicity to bacteria in the cecal microbiome in both in vivo and in vitro models; (5) bile acids directly and rapidly affect bacterial global metabolism including membrane damage, disrupted amino acid, nucleotide, and carbohydrate metabolism; and (6) in vivo, short-term exposure to bile acids significantly affected host metabolism via alterations of the bacterial community...Continue Reading

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Citations

Dec 10, 2020·Gut Microbes·Yuan TianAndrew D Patterson
Jan 6, 2021·Inflammatory Bowel Diseases·Na LiXiaojun Zhuang
Aug 31, 2020·IScience·Qian ZhangGuiyu Wang
Dec 16, 2020·Cellular & Molecular Immunology·Rui WangHerbert Tilg
Feb 14, 2021·International Journal of Molecular Sciences·Maria Chiara di GregorioLuciano Galantini
Apr 1, 2021·Microbes and Infection·Giuliano BayerDana J Philpott
Apr 4, 2021·Microorganisms·Jack W DalyCormac G M Gahan
May 3, 2021·Veterinary Microbiology·Fanzhi KongQiuhong Wang
May 29, 2021·Molecular and Cellular Endocrinology·Victoria Fernández-GarcíaLisardo Boscá
Jun 24, 2021·Seminars in Liver Disease·Huasheng YuWenqin Luo
May 13, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Frans Stellaard, Dieter Lütjohann
Jul 18, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Victoria Fernández-GarcíaLisardo Boscá
Jul 20, 2021·Tissue Barriers·Maria Victoria Fernandez-CantosOscar P Kuipers
Aug 17, 2021·World Journal of Microbiology & Biotechnology·Syarifah Ab RashidMohd Azizan Mohd Noor

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Methods Mentioned

BETA
flow cytometry
NMR
Protein Assay
PCR

Software Mentioned

SIMCA
FlowJo
BLAST
R
MetaMapp
SOM
Cytoscape
Suprahex
GraphPad Prism

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