The Microflora of Chronic Diabetic Foot Ulcers Based on Culture and Molecular Examination: A Descriptive Study

Wound Management & Prevention
Tuhina BanerjeeSomprakas Basu

Abstract

Infection of chronic diabetic foot ulcers (DFUs) is a major concern in patients with diabetes mellitus. This prospective, descriptive study was conducted to evaluate clinical wound parameters and to determine the aerobic and anaerobic microflora of DFUs with 1 or more clinical signs of infection using culture and molecular methods. Patients with a DFU and clinical signs of infection receiving care at a tertiary care hospital in Varanasi, India, were consecutively enrolled. Patient and wound characteristics were assessed, and the cultures obtained were analyzed quantitatively to detect aerobes/facultative anaerobes and by polymerase chain reaction for common anaerobes. If no organisms were found using these methods, sequence analysis of bacterial 16S ribosomal RNA (16SrRNA) was used. Clinical, demographic, and microbial flora variables were compared using the chi-squared test, and predictors of culture results were ascertained using multiple logistic regressions. Forty (40) patients participated. Of those, 30 (75%) had positive culture results with a total of 64 isolates (2.13 isolates/ulcer). The ratio of aerobes to anaerobes was 1.24:1 (35/29); Peptococcus spp was the most frequent isolate (15, 23.4%). Proteobacteria and Firmi...Continue Reading

Citations

Feb 6, 2021·International Wound Journal·Prakhar JaiswalTuhina Banerjee

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