PMID: 2113183Jun 1, 1990Paper

The micronucleus assay as a test for the detection of aneugenic activity

Mutation Research
P VanparysR Temmerman

Abstract

The aim of this work was to determine the usefulness of the micronucleus assay for the detection of aneugenic potential. Chemicals affecting microtubule assembly, i.e., colchicine, vinblastine sulfate and tubulazole, and chemicals affecting targets other than microtubuli, i.e., mitomycin C, cyclophosphamide and miconazole, and the clastogens azathioprine and procarbazine were administered once orally or intraperitoneally to male and female mice. Bone marrow preparations were made at 24, 48 and 72 h after dosing. All the clastogens and aneugens, except miconazole, yielded positive results in the micronucleus test. Measurements of the area of the micronuclei and their distribution clearly showed that the chemicals affecting microtubule assembly produced larger micronuclei than did the clastogens. The pattern of area distribution of the micronuclei found with cyclophosphamide and mitomycin C was between those found for the tubulin inhibitors and the clastogens. These findings indicate that the micronucleus test not only detects chemicals affecting microtubule assembly, but also can discriminate them from clastogens by measurements of the area of the micronuclei.

References

Feb 1, 1975·Mutation Research·W Schmid
Nov 1, 1976·Mutation Research·P Maier, W Schmid
May 1, 1973·Mutation Research·J A Heddle
Jul 1, 1973·Mutation Research·M von Ledebur, W Schmid
Oct 1, 1980·Mutation Research·D BellincampiG P Morpurgo
Jan 1, 1994·Environmental and Molecular Mutagenesis·A M MotimayaR M Kitchin

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Citations

Aug 17, 1996·Mutation Research·P R Turner, W A Denny
Jul 1, 1992·Mutation Research·P VanparysR Marsboom
Jan 1, 1991·Toxicology in Vitro : an International Journal Published in Association with BIBRA·L Migliore, M Nieri
Mar 5, 2016·South Asian Journal of Cancer·Abhimanyu MohantaGadadhar Parida
Dec 19, 2012·Asian Pacific Journal of Cancer Prevention : APJCP·Abdullah Narin, Orta Tuncay
Sep 28, 2010·Environmental and Molecular Mutagenesis·Stephanie CoffingMaik Schuler
Jun 4, 2019·Environmental Science and Pollution Research International·Emine YalçınKültiğin Çavuşoğlu
Jan 1, 1995·Environmental and Molecular Mutagenesis·A ElhajoujiM Kirsch-Volders
Aug 24, 2017·Environmental and Molecular Mutagenesis·Svetlana L AvlasevichStephen D Dertinger

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