The MicroRNA Expression Profiles of Human Temporal Lobe Epilepsy in HS ILAE Type 1

Cellular and Molecular Neurobiology
Chongyang TangZhiguo Lin

Abstract

Temporal lobe epilepsy (TLE) is associated with neurodegeneration, often leading to hippocampal sclerosis (HS). Type 1 HS, which is characterized by severe neuronal loss and gliosis predominantly in regions CA1 and CA4, is the most common subtype and is associated with the best prognosis according to the ILAE classification system. MiRNAs participate in the biological processes underlying many nervous system diseases, including epilepsy. However, the miRNA expression profile of HS ILAE type 1 is not completely understood. A total of 14 patients were identified as having the ILAE subtype, as determined by NeuN immunohistochemistry (ILAE type 1 = 7; no-HS = 7). Next-generation sequencing and reverse transcription polymerase chain reaction technology were used to validate the dysregulated miRNAs. Bioinformatics analysis of the predicted target genes was conducted using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. In total, 1643 mature miRNAs were detected in this study, along with 5 miRNAs that were upregulated and 2 miRNAs that were downregulated in the type 1 group. Bioinformatics analysis showed that 1545 target genes were predicted using the miRDB and Targetscan databases and that these predicted genes s...Continue Reading

References

Jan 16, 2007·Acta Neuropathologica·Ingmar BlümckeMichelle Hildebrandt
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Sep 14, 2011·Nucleic Acids Research·Marc R FriedländerNikolaus Rajewsky
Apr 27, 2012·Cellular and Molecular Life Sciences : CMLS·Anne A KanR Jeroen Pasterkamp
May 9, 2014·Cellular and Molecular Life Sciences : CMLS·Kärt VarendiJaan-Olle Andressoo
Aug 17, 2014·Translational Stroke Research·Sofie Sølvsten SørensenThomas Christensen
Sep 18, 2014·Neuropsychiatric Disease and Treatment·Ludmyla KandrataviciusJoao Pereira Leite
Dec 3, 2014·Seizure : the Journal of the British Epilepsy Association·Meng NaZhiguo Lin
Nov 4, 2015·Frontiers in Molecular Neuroscience·Walid A AlsharafiZhaohui Luo
Feb 19, 2016·Journal of Alzheimer's Disease : JAD·Hsin-Hua LiChih-Li Lin
Nov 12, 2016·Biochemical and Biophysical Research Communications·Rainer SurgesAlexander Pfeifer
Nov 15, 2016·Lancet Neurology·David C HenshallFelix Rosenow
Apr 26, 2017·Molecular Medicine Reports·Junqiang DaiYawen Pan
Nov 28, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Maryam Ghasemi-KasmanMohammad Javan
Nov 28, 2017·Molecular and Cellular Neurosciences·Maryam Ghasemi-KasmanMohammad Javan
Feb 2, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Francesca BalzanoMargherita Maioli

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Methods Mentioned

BETA
light microscopy
RNA Assay
Assay
Chips
PCR
RNA-seq
surgical resection

Software Mentioned

GraphPad Prism
mirDeep2
sRNA
DAVID
custom Perl
Bowtie
Python scripts
- tools cli
cBot Cluster Generation System
DESeq R

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