The microRNA miR-184 regulates the CYP303A1 transcript level to control molting of Locusta migratoria.

Insect Science
Yan-Li WangJian-Zhen Zhang

Abstract

Cytochrome P450 monooxygenases (CYPs) play essential physiological functions in insects. CYP303A1 is highly conserved in insect species studied to date, and shows an indispensable role for adult eclosion in both Locusta migratoria and Drosophila melanogaster. However, how CYP303A1 is regulated to control insect developmental processes remains uninvestigated. In this study, we discovered functional binding sites for miR-184 in the coding sequence of LmCYP303A1. The luciferase reporter assay showed that miR-184 could target LmCYP303A1 and regulate its expression in vitro. Furthermore, overexpression of miR-184 through microinjection of agomir to locusts reduced the transcripts of LmCYP303A1 and led to abnormal molting, which is similar to the phenotype of silencing LmCYP303A1 by direct injection of dsLmCYP303A1 to locusts. Meanwhile, down-regulation of miR-184 by injection of antagomir increased the LmCYP303A1 transcript and caused molting defects. These findings suggested that miR-184 could target LmCYP303A1 to regulate the molting process in L. migratoria, which might be considered as a novel target for pest control.

References

Feb 24, 2001·Genome Biology·D Werck-Reichhart, R Feyereisen
Aug 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·James T WarrenLawrence I Gilbert
Nov 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Anna PetrykMichael B O'Connor
Mar 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·C HelvigR Feyereisen
Aug 26, 2004·Mechanisms of Development·Aarron T Willingham, Thomas Keil
Dec 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Le KangJun Yu
Aug 24, 2006·Annual Review of Entomology·Xianchun LiMay R Berenbaum
Jul 22, 2009·Developmental Cell·Nicola IovinoUlrike Gaul
Dec 3, 2009·Human Genomics·David R Nelson
Mar 27, 2010·Molecular Biology Reports·Ping LiLiudi Yuan
Jul 28, 2010·Nature Reviews. Genetics·Jacek KrolWitold Filipowicz
May 1, 2012·Cell·Margaret S Ebert, Phillip A Sharp
Jun 13, 2012·Biological & Pharmaceutical Bulletin·Masatoshi Iga, Hiroshi Kataoka
Aug 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yue QiuRené Feyereisen
Oct 30, 2012·Insect Biochemistry and Molecular Biology·Sassan Asgari
Apr 17, 2013·Biotechnology and Applied Biochemistry·Takaaki Daimon, Tetsuro Shinoda
Jul 13, 2014·Insect Biochemistry and Molecular Biology·Lin LingAnjiang Tan
Aug 8, 2015·Current Opinion in Insect Science·Keira J LucasAlexander S Raikhel
Aug 27, 2015·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Jianjian PengLiudi Yuan
Sep 4, 2015·Scientific Reports·Yanli WangLe Kang
Jul 22, 2016·Proceedings of the National Academy of Sciences of the United States of America·Vasileia BalabanidouJohn Vontas
Sep 22, 2016·Parasitology Research·Mengmeng TianChangliang Zhu
Oct 1, 2017·Aging Cell·Christi M Gendron, Scott D Pletcher
Aug 20, 2019·Insect Biochemistry and Molecular Biology·Lixian WuSheng Li

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Citations

Feb 9, 2021·Insect Biochemistry and Molecular Biology·Xiaoxi ChenQiangqiang Jia

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