The MicroRNA319d/TCP10 Node Regulates the Common Bean - Rhizobia Nitrogen-Fixing Symbiosis

Frontiers in Plant Science
José Á Martín-RodríguezGeorgina Hernández

Abstract

Micro-RNAs from legume plants are emerging as relevant regulators of the rhizobia nitrogen-fixing symbiosis. In this work we functionally characterized the role of the node conformed by micro-RNA319 (miR319) - TEOSINTE BRANCHED/CYCLOIDEA/PCF (TCP) transcription factor in the common bean (Phaseolus vulgaris) - Rhizobium tropici symbiosis. The miR319d, one of nine miR319 isoforms from common bean, was highly expressed in root and nodules from inoculated plants as compared to roots from fertilized plants. The miR319d targets TCP10 (Phvul.005G067950), identified by degradome analysis, whose expression showed a negative correlation with miR319d expression. The phenotypic analysis of R. tropici-inoculated composite plants with transgenic roots/nodules overexpressing or silencing the function of miR319d demonstrated the relevant role of the miR319d/TCP10 node in the common bean rhizobia symbiosis. Increased miR319d resulted in reduced root length/width ratio, increased rhizobial infection evidenced by more deformed root hairs and infection threads, and decreased nodule formation and nitrogenase activity per plant. In addition, these plants with lower TCP10 levels showed decreased expression level of the jasmonic acid (JA) biosynthetic...Continue Reading

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Citations

Mar 13, 2020·Journal of Experimental Botany·Nhung T HoangGary Stacey
Dec 15, 2020·Physiologia Plantarum·Jitendra KumarKadambot H M Siddique
Apr 11, 2021·Journal of Integrative Plant Biology·Rongna WangZhongming Fang
Jun 25, 2021·Frontiers in Plant Science·Litzy AyraGeorgina Hernández

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Methods Mentioned

BETA
transgenic
RNAseq
PCR
light microscopy
sRNA-seq
RNA-seq
immunoprecipitation

Software Mentioned

MAFFT
GraphPad Prism
Clover

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