The MicroRNA828/MYB12 Module Mediates Bicolor Pattern Development in Asiatic Hybrid Lily (Lilium spp.) Flowers

Frontiers in Plant Science
Masumi Yamagishi, Moeko Sakai

Abstract

Some Asiatic hybrid lily cultivars develop bicolor tepals, which consist of anthocyanin-pigmented upper halves and un-pigmented lower halves. MYB12, a subgroup 6 member of R2R3-MYB that positively regulates anthocyanin biosynthesis, is downregulated in the lower halves. However, MYB12 is usually expressed over entire tepal regions in numerous lily cultivars. Why MYB12 of bicolor cultivars exhibits variable expression spatially in a single tepal remains unclear. Since the lily MYB12 mRNA harbored a binding site for microRNA828 (miR828), the involvement of miR828 in variable spatial accumulation of MYB12 transcripts was evaluated. We analyzed the cleavage of MYB12 mRNA, mature miR828 accumulation, and MYB12 transcript-derived siRNA generation (microRNA-seq). In the bicolor tepals, mature miR828 was more highly accumulated in the lower halves than in the upper halves, and miR828-directed cleavage of MYB12 transcripts was observed predominantly in the lower halves. Moreover, the cleavage triggered the production of secondary siRNA from MYB12 transcripts, and the siRNAs were accumulated predominantly in the lower halves. Consequently, miR828 suppressed MYB12 transcript accumulation in the white region, and the miR828/MYB12 module pa...Continue Reading

References

Oct 13, 2001·Current Opinion in Plant Biology·R StrackeB Weisshaar
Sep 1, 2005·Genes & Development·Manabu YoshikawaR Scott Poethig
Dec 22, 2006·Genes & Development·Ramya RajagopalanDavid P Bartel
Oct 13, 2007·Plant Methods·Erika Varkonyi-GasicRoger P Hellens
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Jan 25, 2011·Annals of Botany·M D Bennett, I J Leitch
May 3, 2011·Plant Molecular Biology·Qing-Jun LuoChristopher D Rock
Mar 13, 2012·The Plant Cell·Padubidri V ShivaprasadDavid C Baulcombe
Jun 19, 2012·Genome Biology·Rui XiaZongrang Liu
Jul 17, 2012·Plant Science : an International Journal of Experimental Plant Biology·Yun-Song LaiMasumi Yamagishi
Jan 9, 2013·The New Phytologist·Beverley J GloverSamuel F Brockington
Apr 2, 2013·Molecular Genetics and Genomics : MGG·Masaki MomoseYoshihiro Ozeki
Jul 9, 2013·Essays in Biochemistry·Tamas Dalmay
Feb 24, 2015·Journal of Experimental Botany·Baohong Zhang
Mar 13, 2015·Plant Signaling & Behavior·Nick W AlbertKathy E Schwinn
May 22, 2016·Plant Physiology·Deepika SharmaPrabodh Kumar Trivedi
Oct 17, 2017·Trends in Plant Science·Marco D'ArioMinsung Kim
Jan 13, 2018·Plant Biotechnology Journal·Pingchuan DengLiang Wu
Apr 24, 2018·Breeding Science·Agnieszka Marasek-CiolakowskaKeiichi Okazaki
May 3, 2018·Nucleic Acids Research·Xinbin DaiPatrick Xuechun Zhao
Jul 27, 2018·The Plant Journal : for Cell and Molecular Biology·Stefano GattolinLaura Rossini
Jan 9, 2019·Frontiers in Plant Science·Nicola BonarCsaba Hornyik
Apr 12, 2019·Frontiers in Plant Science·Junli WangGuodong Ren
May 31, 2019·Journal of Experimental Botany·Varsha TirumalaiPadubidri V Shivaprasad
Feb 27, 2020·The Plant Journal : for Cell and Molecular Biology·Kazunori KuriyamaToshiyuki Fukuhara
Mar 12, 2020·Annual Review of Plant Biology·Róisín Fattorini, Beverley J Glover

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Citations

Aug 28, 2021·International Journal of Molecular Sciences·Emil KhusnutdinovElena Mikhaylova

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Datasets Mentioned

BETA
LC569960
LC569961
DRA010576

Methods Mentioned

BETA
microRNA-seq
PCR
Assay
reverse transcription PCR

Software Mentioned

R
Cutadapt
Bowtie
Clustal
Fastq
Lollypop
join
psRNATarget : A Plant Small RNA Target Analysis Server

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