The microRNAs miR-373 and miR-520c promote tumour invasion and metastasis

Nature Cell Biology
Qihong HuangReuven Agami

Abstract

MicroRNAs (miRNAs) are single-stranded, noncoding RNAs that are important in many biological processes. Although the oncogenic and tumour-suppressive functions of several miRNAs have been characterized, the role of miRNAs in mediating tumour metastasis was addressed only recently and still remains largely unexplored. To identify potential metastasis-promoting miRNAs, we set up a genetic screen using a non-metastatic, human breast tumour cell line that was transduced with a miRNA-expression library and subjected to a trans-well migration assay. We found that human miR-373 and miR-520c stimulated cancer cell migration and invasion in vitro and in vivo, and that certain cancer cell lines depend on endogenous miR-373 activity to migrate efficiently. Mechanistically, the migration phenotype of miR-373 and miR-520c can be explained by suppression of CD44. We found significant upregulation of miR-373 in clinical breast cancer metastasis samples that correlated inversely with CD44 expression. Taken together, our findings indicate that miRNAs are involved in tumour migration and invasion, and implicate miR-373 and miR-520c as metastasis-promoting miRNAs.

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References

Mar 4, 2000·International Journal of Cancer. Journal International Du Cancer·S H ChoiK K Tanabe
Mar 23, 2002·Science·Thijn R BrummelkampReuven Agami
Jun 28, 2002·Cancer Metastasis Reviews·E B JaegerC W Rinker-Schaeffer
Jan 4, 2003·Nature Reviews. Molecular Cell Biology·Helmut PontaPeter A Herrlich
Jan 8, 2003·Pathology Oncology Research : POR·Mousa KhoursheedA Al-Bader
Oct 18, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Motoko SasakiYasuni Nakanuma
Jun 24, 2004·Nature Reviews. Genetics·Lin He, Gregory J Hannon
Sep 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Svetlana MukhinaPeter E Lobie
Sep 17, 2004·Nature·Victor Ambros
Dec 21, 2004·Cancer Cell·Peiwen FeiWafik S El-Deiry
Mar 5, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yuri TakahashiShinzaburo Noguchi
Apr 26, 2005·World Journal of Gastroenterology : WJG·Jian-Lin RenHua-Xiu Shi
May 4, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Leslie K DiazMichael Z Gilcrease
Jun 10, 2005·Nature·Lin HeScott M Hammond
Jun 21, 2005·Nature Genetics·Isaac BentwichZvi Bentwich
Jul 13, 2005·Cell·Witold Filipowicz
Aug 3, 2005·Cancer Research·Jose I LopezJoyce A Schroeder
Oct 15, 2005·Development·Tingting Du, Phillip D Zamore
Feb 24, 2006·British Journal of Cancer·H-W Hwang, J T Mendell
Mar 1, 2006·Current Opinion in Genetics & Development·Richard W Carthew
Mar 15, 2006·Cell·Ronald H A Plasterk
Mar 25, 2006·Nature Reviews. Cancer·Aurora Esquela-Kerscher, Frank J Slack
Aug 8, 2006·Nature Medicine·Patricia S Steeg
Nov 18, 2006·Cell·Gaorav P Gupta, Joan Massagué
Apr 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kiranmai GumireddyQihong Huang

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Citations

Aug 29, 2012·Cellular and Molecular Life Sciences : CMLS·Huiping Liu
Jan 15, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Achim Aigner
Apr 16, 2009·Analytical and Bioanalytical Chemistry·Kyle A Cissell, Sapna K Deo
Nov 26, 2008·World Journal of Surgery·Min LiChangyi Chen
Aug 22, 2009·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Nicola ValeriMuller Fabbri
Nov 19, 2009·Breast Cancer Research and Treatment·Rongxi YangBarbara Burwinkel
Jan 8, 2010·Breast Cancer Research and Treatment·Selma MasriShiuan Chen
Dec 17, 2009·Cancer Metastasis Reviews·Sylvia K Shenouda, Suresh K Alahari
Feb 5, 2010·Cancer Metastasis Reviews·Liqin Du, Alexander Pertsemlidis
Oct 13, 2010·Cancer Metastasis Reviews·Ming ShiNing Guo
Jun 12, 2012·Cancer Metastasis Reviews·Jinchun Yan, Qihong Huang
Jun 12, 2012·Cancer Metastasis Reviews·Jinsong Zhang, Li Ma
Mar 13, 2012·Clinical & Experimental Metastasis·Mian Ling ChenXiao Kang Wang
May 26, 2010·Journal of Mammary Gland Biology and Neoplasia·Josephine A WrightGregory J Goodall
Mar 31, 2011·Journal of Mammary Gland Biology and Neoplasia·Jenny G ParvaniWilliam P Schiemann
Feb 2, 2012·Journal of Mammary Gland Biology and Neoplasia·Scott Valastyan
Feb 22, 2012·Journal of Mammary Gland Biology and Neoplasia·Hai-Long Piao, Li Ma
Jun 22, 2010·Molecular Biology Reports·Nuray VarolSinan Sozen
Aug 9, 2011·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Fanfei KongGang Chen
Aug 14, 2012·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Peng XieYouhuang Liu
Apr 18, 2009·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Imran BhattiMichael Larvin
Jan 7, 2011·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Xiao-Feng LeGeorge A Calin
Oct 12, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Rongfei HanQiuping Zhang
Dec 15, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Weijie ChenXiao Yan Zhong
Oct 1, 2011·Cellular Oncology (Dordrecht)·Luisa MontaniniMaria Serena Benassi
Aug 21, 2013·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Bo GongLei Zhang
Dec 12, 2012·Cancer Letters·Nicholas C D'AmatoJennifer K Richer
Jun 16, 2009·Cell·Scott ValastyanRobert A Weinberg
Aug 7, 2012·Asian Journal of Andrology·Sung Gu KangChul Hwan Kim
Mar 31, 2010·Nature Biotechnology·Li MaRobert A Weinberg
Oct 11, 2008·Nature Biotechnology·Avak KahvejianJohn F Thompson
Feb 23, 2010·Nature Cell Biology·Yeesim Khew-Goodall, Gregory J Goodall
May 6, 2008·Nature Cell Biology·Eric A Miska
Feb 23, 2010·Nature Cell Biology·Li MaRobert A Weinberg
Jun 4, 2013·Nature Cell Biology·Nora Pencheva, Sohail F Tavazoie
Dec 29, 2013·Nature Genetics·Tenley C Archer, Scott L Pomeroy
Aug 5, 2008·Nature Immunology·Noam Stern-GinossarOfer Mandelboim
Mar 6, 2009·Nature Reviews. Cancer·Milena S NicolosoGeorge A Calin
Aug 9, 2011·Nature Reviews. Cancer·Marieke van KouwenhoveReuven Agami

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