The Microstructure, Proteomics and Crystallization of the Limpet Teeth

Proteomics
Yadong WangRongqing Zhang

Abstract

Limpets are marine mollusks that use mineralized teeth, one of the hardest and strongest biomaterials, to feed on algae on intertidal rocks. However, most of studies only focus on the ultrastructure and chemical composition of the teeth while the molecular information is largely unknown, limiting our understanding of this unique and fundamental biomineralization process. The study investigates the microstructure, proteomics, and crystallization in the teeth of limpet Cellana toreuma. It is found that the limpets formed alternatively tricuspid teeth and unicuspid teeth. Small nanoneedles are densely packed at the tips or leading regions of the cusps. In contrast, big nanoneedles resembling chemically synthesized goethite are loosely packed in the trailing regions of the cusps. Proteins extracted from the whole radula, such as ferritin, peroxiredoxin, arginine kinase, GTPase-Rabs, and clathrin, are identified by proteomics. A goethite-binding experiment coupled with proteomics and RNA-seq highlights six chitin-binding proteins (CtCBPs). Furthermore, the extracted proteins from the cusps of radula or the framework chitin induce packing of crystals and possibly affect crystal polymorphs in vitro. This study provides insight into th...Continue Reading

References

Jan 1, 1969·Zeitschrift für Zellforschung und mikroskopische Anatomie·N W RunhamR C Wayte
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·H MiyamotoA Matsushiro
May 20, 1998·Nature Structural Biology·H J ChoiS E Ryu
Jun 2, 2001·Genome Biology·H Stenmark, V M Olkkonen
Feb 20, 2004·Journal of Colloid and Interface Science·Marek KosmulskiJarl B Rosenholm
Jul 28, 2005·The Journal of Biological Chemistry·Gen HeAnne George
Mar 31, 2007·Physical Chemistry Chemical Physics : PCCP·I V ChernyshovaA S Madden
May 18, 2007·Progress in Biophysics and Molecular Biology·Ming Ren Yen, Milton H Saier
Mar 11, 2008·Journal of Veterinary Medical Education·Helen M ChapmanDavid J Murphy
Dec 25, 2008·Journal of Morphology·Jeremy A ShawPeta L Clode
Aug 15, 2009·Science·Michio SuzukiHiromichi Nagasawa
Sep 4, 2009·Journal of the Royal Society, Interface·Markus RüggebergThomas Speck
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
Dec 14, 2011·Journal of the Royal Society, Interface·Dun Lu, Asa H Barber
Dec 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Benjamin MarieCaroline Montagnani
Jun 15, 2013·Molecular Biology and Evolution·Paula Ramos-SilvaFrédéric Marin
Sep 10, 2014·Angewandte Chemie·Lyle M GordonDerk Joester
Feb 20, 2015·Journal of the Royal Society, Interface·Asa H BarberNicola M Pugno
Jun 9, 2015·Nature Chemical Biology·YerPeng TanJ Herbert Waite
Oct 4, 2015·Journal of Structural Biology·Tina Ukmar-GodecDamien Faivre
Nov 27, 2015·Scientific Reports·Chuang LiuRongqing Zhang
Oct 17, 2017·Current Biology : CB·Tali MassPaul G Falkowski
May 5, 2018·Science Advances·Dimitra AthanasiadouMarc D McKee

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Citations

Feb 18, 2021·Biological Reviews of the Cambridge Philosophical Society·Peter A DaveyNick Aldred
Mar 3, 2021·Journal of Proteomics·Chuang Liu, Rongqing Zhang

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