The microtubule-based motor Kar3 and plus end-binding protein Bim1 provide structural support for the anaphase spindle

The Journal of Cell Biology
Melissa K GardnerKerry Bloom

Abstract

In budding yeast, the mitotic spindle is comprised of 32 kinetochore microtubules (kMTs) and approximately 8 interpolar MTs (ipMTs). Upon anaphase onset, kMTs shorten to the pole, whereas ipMTs increase in length. Overlapping MTs are responsible for the maintenance of spindle integrity during anaphase. To dissect the requirements for anaphase spindle stability, we introduced a conditionally functional dicentric chromosome into yeast. When centromeres from the same sister chromatid attach to opposite poles, anaphase spindle elongation is delayed and a DNA breakage-fusion-bridge cycle ensues that is dependent on DNA repair proteins. We find that cell survival after dicentric chromosome activation requires the MT-binding proteins Kar3p, Bim1p, and Ase1p. In their absence, anaphase spindles are prone to collapse and buckle in the presence of a dicentric chromosome. Our analysis reveals the importance of Bim1p in maintaining a stable ipMT overlap zone by promoting polymerization of ipMTs during anaphase, whereas Kar3p contributes to spindle stability by cross-linking spindle MTs.

References

Jul 1, 1987·Molecular and Cellular Biology·A Hill, K Bloom
Jun 1, 1995·The Journal of Cell Biology·M WineyJ R McIntosh
Jan 1, 1996·Journal of Structural Biology·J R KremerJ R McIntosh
Jul 25, 1997·Science·A F StraightA W Murray
Jan 27, 1998·Journal of Structural Biology·D N Mastronarde
Jun 3, 1999·The Journal of Cell Biology·J S TirnauerD Pellman
Jul 31, 1999·The Journal of Cell Biology·X ZengW S Saunders
Jun 21, 2002·Methods in Enzymology·Eileen T O'TooleDavid N Mastronarde
May 29, 2003·Biophysical Journal·Brian L SpragueDavid J Odde
Jan 14, 2004·Molecular Biology of the Cell·Jeffrey N MolkKerry Bloom
Aug 9, 2005·Current Biology : CB·Lisa R SproulSusan P Gilbert
Mar 15, 2006·The Journal of Cell Biology·Jessica D Tytell, Peter K Sorger
Dec 27, 2006·Methods : a Companion to Methods in Enzymology·Melissa K GardnerKerry Bloom

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Citations

Oct 24, 2009·Nature Reviews. Molecular Cell Biology·Kristen J Verhey, Jennetta W Hammond
Jun 18, 2011·The Journal of Biological Chemistry·Chun Ju ChenSusan P Gilbert
Sep 15, 2012·The Journal of Biological Chemistry·Chun Ju ChenSusan P Gilbert
Feb 6, 2009·Physical Biology·B RubinsteinA Mogilner
Aug 24, 2013·Molecular Biology of the Cell·Elena NazarovaJackie Vogel
Aug 21, 2013·PloS One·Rosa R Mouriño-PérezMichael Freitag
Apr 12, 2012·Genetics·Mark Winey, Kerry Bloom
Jul 8, 2010·Annual Review of Cell and Developmental Biology·Gohta Goshima, Jonathan M Scholey
Oct 12, 2013·Genes & Development·Seoyoung KimDean S Dawson
May 13, 2010·Cellular and Molecular Bioengineering·Melissa K GardnerDavid J Odde
Dec 2, 2009·The Journal of Cell Biology·Thibault CourtheouxSylvie Tournier
Aug 12, 2009·The Journal of Cell Biology·Tomasz ZimniakStefan Westermann
Jun 29, 2011·The Journal of Cell Biology·Andrew D StephensKerry Bloom
Jun 27, 2012·The Journal of Cell Biology·Katherine C RankIvan Rayment
Aug 28, 2015·Molecular Biology of the Cell·Sabyasachi SutradharKaustuv Sanyal
Nov 18, 2010·Molecular Systems Biology·Benjamin VanderSluisChad L Myers
Jul 4, 2012·Bioarchitecture·Christina ThiedeChristoph F Schmidt
Dec 23, 2009·Journal of Structural Biology·Julia CopeAndreas Hoenger
Apr 24, 2012·Current Biology : CB·Tomasz ZimniakStefan Westermann
Apr 10, 2008·Current Biology : CB·Marcel E Janson, Phong T Tran
Jan 4, 2013·Molecular Biology of the Cell·Daniel RichmondYanchang Wang
Nov 10, 2012·Biochemical and Biophysical Research Communications·Caroline DelormeJohn S Allingham
Aug 11, 2017·The Journal of Cell Biology·Yili ZhuWei-Lih Lee
Jul 31, 2019·Nature Cell Biology·Javier Manzano-LópezFernando Monje-Casas
Oct 1, 2020·Molecular Biology of the Cell·Ezekiel C ThomasJeffrey K Moore
Sep 28, 2012·Journal of Cell Science·Romain GibeauxClaude Antony

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Methods Mentioned

BETA
electrophoresis
electron tomography
PCR
electron microscopy

Software Mentioned

Excel
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