The MinDE system is a generic spatial cue for membrane protein distribution in vitro

Nature Communications
Beatrice RammPetra Schwille

Abstract

The E. coli MinCDE system has become a paradigmatic reaction-diffusion system in biology. The membrane-bound ATPase MinD and ATPase-activating protein MinE oscillate between the cell poles followed by MinC, thus positioning the main division protein FtsZ at midcell. Here we report that these energy-consuming MinDE oscillations may play a role beyond constraining MinC/FtsZ localization. Using an in vitro reconstitution assay, we show that MinDE self-organization can spatially regulate a variety of functionally completely unrelated membrane proteins into patterns and gradients. By concentration waves sweeping over the membrane, they induce a direct net transport of tightly membrane-attached molecules. That the MinDE system can spatiotemporally control a much larger set of proteins than previously known, may constitute a MinC-independent pathway to division site selection and chromosome segregation. Moreover, the here described phenomenon of active transport through a traveling diffusion barrier may point to a general mechanism of spatiotemporal regulation in cells.

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Citations

May 28, 2019·BMC Biology·Simon KretschmerPetra Schwille
Nov 10, 2018·Microbial Biotechnology·Beatrice Ramm, Petra Schwille
Jul 19, 2019·Cellular and Molecular Life Sciences : CMLS·Beatrice RammPetra Schwille
May 18, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jaime Agudo-CanalejoRamin Golestanian
Jul 11, 2020·Advanced Biosystems·Daniel HürtgenPetra Schwille

Methods Mentioned

BETA
gel filtration
PCR
Fluorescence
gel-filtration
affinity purification
electrophoresis

Related Concepts

FtsZ protein, Bacteria
MinD protein, E coli
MinE protein, E coli
DNA-dependent ATPase
Striadyne
Bacterial Proteins
Cytoskeletal Proteins
DNA, Double-Stranded
Alkalescens-Dispar Group
Cell Surface Proteins

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