The Mini-Organo: A rapid high-throughput 3D coculture organotypic assay for oncology screening and drug development.

Cancer Reports
Jessica L ChittyThomas R Cox

Abstract

The use of in vitro cell cultures is a powerful tool for obtaining key insights into the behaviour and response of cells to interventions in normal and disease situations. Unlike in vivo settings, in vitro experiments allow a fine-tuned control of a range of microenvironmental elements independently within an isolated setting. The recent expansion in the use of three-dimensional (3D) in vitro assays has created a number of representative tools to study cell behaviour in a more physiologically 3D relevant microenvironment. Complex 3D in vitro models that can recapitulate human tissue biology are essential for understanding the pathophysiology of disease. The development of the 3D coculture collagen contraction and invasion assay, the "organotypic assay," has been widely adopted as a powerful approach to bridge the gap between standard two-dimensional tissue culture and in vivo mouse models. In the cancer setting, these assays can then be used to dissect how stromal cells, such as cancer-associated fibroblasts (CAFs), drive extracellular matrix (ECM) remodelling to alter cancer cell behaviour and response to intervention. However, to date, many of the published organotypic protocols are low-throughput, time-consuming (up to sever...Continue Reading

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Citations

Feb 4, 2021·International Journal of Molecular Sciences·Giada BassiMonica Montesi
Oct 30, 2021·Frontiers in Digital Health·Rodrigo CurvelloDaniela Loessner

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Software Mentioned

FIJI
Organo
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QuPath
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