The minimum inhibitory concentration (MIC) assay with Escherichia coli: An early tier in the environmental hazard assessment of nanomaterials?

Ecotoxicology and Environmental Safety
J VassalloRichard D Handy

Abstract

There are now over a thousand nano-containing products on the market and the antibacterial properties of some nanomaterials has created interest in their use as cleaning agents, biocides and disinfectants. Engineered nanomaterials (ENMs) are being released into the environment and this raises concerns about their effects on microbes in the receiving ecosystems. This study evaluated the bacterial toxicity of a wide range of nanomaterials with different surface coatings on Escherichia coli K-12 MG1655. The minimum inhibitory concentration (MIC) assay, which quantifies the threshold for growth inhibition in suspensions of bacteria, was used to rank the toxicity of silver (Ag), cupric oxide (CuO), cadmium telluride (CdTe) quantum dots, titanium dioxide (TiO2), nanodiamonds and multi-walled carbon nanotubes (MWCNTs). Bacteria were exposed for 12 h at 37 °C to a dilution series of the test suspensions in 96-well plates. The precision and accuracy of the method was good with coefficients of variation < 10%. In terms of the measured MIC values, the toxicity order of the ENMs was as follows: CdTe quantum dots ammonium-coated, 6 mg L-1 > Ag nanoparticles, 12 mg L-1 > CdTe quantum dots carboxylate-coated, 25 mg L-1 > CdTe quantum dots pol...Continue Reading

Citations

Jul 7, 2019·Particle and Fibre Toxicology·Marit IlvesHarri Alenius
Oct 10, 2020·Journal of Applied Toxicology : JAT·Xiaoquan Huang, Meng Tang
Mar 2, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Nesreen A FatthallahMohamed A Shenashen
May 25, 2021·Advanced Science·Pia A S KinaretHarri Alenius
Aug 19, 2021·Journal of Clinical Laboratory Analysis·Chuntian QuanJianjiang Zhao

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