The minotaur proteome: avoiding cross-species identifications deriving from bovine serum in cell culture models

Proteomics
Jakob BunkenborgHenrik Molina

Abstract

Cell culture is a fundamental tool in proteomics where mammalian cells are cultured in vitro using a growth medium often supplemented with 5-15% FBS. Contamination by bovine proteins is difficult to avoid because of adherence to the plastic vessel and the cultured cells. We have generated peptides from bovine serum using four sample preparation methods and analyzed the peptides by high mass accuracy LC-MS/MS. Distinguishing between bovine and human peptides is difficult because of a considerable overlap of identical tryptic peptide sequences. Pitfalls in interpretation, different database search strategies to minimize erroneous identifications and an augmented contaminant database are presented.

References

Dec 19, 2002·Molecular & Cellular Proteomics : MCP·N Leigh Anderson, Norman G Anderson
Jun 29, 2004·Proteomics·Debora DumontJohan Robben
Jul 20, 2006·Molecular & Cellular Proteomics : MCP·Patric HörthChristian Wenz
Feb 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Henrik MolinaAkhilesh Pandey
Jun 1, 2007·Nature Methods·Søren SchandorffMatthias Mann
Nov 13, 2008·Cytotechnology·Joanne KeenanMartin Clynes

❮ Previous
Next ❯

Citations

Jul 4, 2013·Journal of Proteome Research·Jakob BunkenborgHenrik Molina
Oct 26, 2013·Nucleic Acids Research·Rosa Viñas-CastellsVíctor M Díaz
Sep 25, 2012·Journal of Proteomics·Marie-Catherine Le BihanGillian Butler-Browne
Nov 18, 2015·The Journal of Cell Biology·Alexandra GrippaPedro Carvalho
Nov 3, 2015·Nature·Ayuko HoshinoDavid Lyden
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Manish P Ponda, Jan L Breslow
Jan 7, 2017·PLoS Pathogens·Melody M H LiMargaret R MacDonald
Jan 9, 2016·Scientific Reports·Ana Sofia CarvalhoRune Matthiesen
Apr 4, 2017·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Sarah BatschkusNicolai Miosge
Jul 15, 2017·Cell Death and Differentiation·Sandra Segura-BayonaTravis H Stracker
Dec 1, 2017·The Journal of Cell Biology·Vitor TeixeiraPedro Carvalho
Jul 10, 2019·Journal of the American Society for Mass Spectrometry·Jeannie M CamarilloNeil L Kelleher
May 24, 2019·Journal of Virology·Melody M H LiMargaret R MacDonald
Mar 12, 2019·Science Advances·Sergi ArandaLuciano Di Croce
Aug 6, 2014·Journal of Proteome Research·Vyacheslav AkimovBlagoy Blagoev

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.