The miR-26b-5p/KPNA2 Axis Is an Important Regulator of Burkitt Lymphoma Cell Growth

Cancers
Fubiao NiuJoost Kluiver

Abstract

The expression of several microRNAs (miRNAs) is known to be changed in Burkitt lymphoma (BL), compared to its normal counterparts. Although for some miRNAs, a role in BL was demonstrated, for most of them, their function is unclear. In this study, we aimed to identify miRNAs that control BL cell growth. Two BL cell lines were infected with lentiviral pools containing either 58 miRNA inhibitors or 44 miRNA overexpression constructs. Eighteen constructs showed significant changes in abundance over time, indicating that they affected BL growth. The screening results were validated by individual green fluorescent protein (GFP) growth competition assays for fifteen of the eighteen constructs. For functional follow-up studies, we focused on miR-26b-5p, whose overexpression inhibited BL cell growth. Argonaute 2 RNA immunoprecipitation (Ago2-IP) in two BL cell lines revealed 47 potential target genes of miR-26b-5p. Overlapping the list of putative targets with genes showing a growth repression phenotype in a genome-wide CRISPR/Cas9 knockout screen, revealed eight genes. The top-5 candidates included EZH2, COPS2, KPNA2, MRPL15, and NOL12. EZH2 is a known target of miR-26b-5p, with oncogenic properties in BL. The relevance of the latter ...Continue Reading

References

Jan 15, 1976·International Journal of Cancer. Journal International Du Cancer·L ZechG Klein
Nov 1, 1958·The British Journal of Surgery·D BURKITT
Dec 11, 2007·Nature Genetics·Tsung-Cheng ChangJoshua T Mendell
Sep 8, 2009·Nucleic Acids Research·Lu Ping TanAnke van den Berg
Mar 25, 2010·British Journal of Haematology·Jan-Lukas RobertusAnke van den Berg
Jan 13, 2012·PloS One·Joost KluiverAnke van den Berg
Jul 26, 2012·Blood Cancer Journal·L Di LisioN Martínez
Aug 1, 2012·Oncology Letters·Jie Gao, Qing-Guang Liu
Jan 9, 2013·Methods in Molecular Biology·Joost KluiverAnke van den Berg
Feb 5, 2014·Cold Spring Harbor Perspectives in Medicine·Roland SchmitzLouis M Staudt
Jul 16, 2014·Nature Reviews. Genetics·Jean Hausser, Mihaela Zavolan
Feb 19, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Melanie WinkleJoost Kluiver
Aug 13, 2015·ELife·Vikram AgarwalDavid P Bartel
Apr 6, 2016·Annals of Hematology·Marcela Cristina RobainaClaudete Esteves Klumb
Apr 23, 2017·The American Journal of Pathology·Ye YuanAnke van den Berg
Jun 29, 2017·International Journal of Cancer. Journal International Du Cancer·Sara LaudatoHeike Allgayer
Oct 27, 2017·Nucleic Acids Research·Daniel D ScottMarlene Oeffinger
Dec 5, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Ke WeiYijiang Chen
Dec 15, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Gang LiYawei Yuan
Aug 18, 2018·Journal of Experimental & Clinical Cancer Research : CR·Jie LiTao Xin
Sep 6, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Ye YuanAnke van den Berg

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Citations

May 25, 2021·Frontiers in Oncology·Yangyang ZengYunfeng Zhou
Jun 3, 2021·Cancers·Marta Cuenca, Victor Peperzak
May 17, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Na ZhaoBo Zhang

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Methods Mentioned

BETA
PCR
xenograft
transfect
transfection
PCRs
flow cytometry
Assay

Software Mentioned

SAM
Light Cycler 480
SPSS
BWA
Pearl script
Python script
MATLAB
Ensembl
TargetScan

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