The miR 495-UBE2C-ABCG2/ERCC1 axis reverses cisplatin resistance by downregulating drug resistance genes in cisplatin-resistant non-small cell lung cancer cells.

EBioMedicine
Jiwei GuoSichuan Xi

Abstract

Cisplatin (DDP) resistance has become the leading cause of mortality in non-small cell lung cancer (NSCLC). miRNA dysregulation significantly contributes to tumor progression. In this study, we found that miR-495 was significantly downregulated in lung cancer tissue specimens. This study aimed to elucidate the functions, direct target genes, and molecular mechanisms of miR-495 in lung cancer. miR-495 downregulated its substrate UBE2C through direct interaction with UBE2C 3'- untranslated region. UBE2C is a proto-oncogene activated in lung cancer; however, its role in chemotherapeutic resistance is unclear. Herein, UBE2C expression levels were higher in DDP-resistant NSCLC cells; this was associated with the proliferation, invasion, and DDP resistance in induced cisplatin-resistant NSCLC cells. Furthermore, epithelial-mesenchymal transitions (EMT) contributed to DDP resistance. Moreover, UBE2C knockdown downregulated vimentin. In contrast, E-cadherin was upregulated. Importantly, miR-495 and UBE2C were associated with cisplatin resistance. We attempted to evaluate their effects on cell proliferation and cisplatin resistance. We also performed EMT, cell migration, and invasion assays in DDP-resistant NSCLC cells overexpressing mi...Continue Reading

Citations

Mar 22, 2019·The Kaohsiung Journal of Medical Sciences·Jin-Guo YuMin-Hua Shi
Oct 15, 2018·Journal of Cellular Physiology·Feng ZhaoRui-Peng Jia
Sep 10, 2020·Signal Transduction and Targeted Therapy·Huibin SongChang Zou
Feb 6, 2020·Clinical and Translational Medicine·Estanislao NavarroMiguel Hueso
May 14, 2020·Clinical and Experimental Medicine·Ilgiz GareevShiguang Zhao
Jan 1, 2020·Journal of Oncology·Yuan-Xiang ShiXin-Yu Song
Jun 4, 2019·Frontiers in Genetics·Johora HannaJannet Kocerha
Jan 11, 2021·Experimental and Molecular Pathology·Mohammad TaheriMarcel E Dinger
Aug 25, 2019·Clinica Chimica Acta; International Journal of Clinical Chemistry·Seyed Mohammad HosseiniFarhad Jadidi-Niaragh
Jan 29, 2021·Cancer Communications·Guangtao PanShenglan Yang
Feb 26, 2021·Pathology, Research and Practice·Soudeh Ghafouri-FardMohammad Taheri
Jul 15, 2021·Pathology Oncology Research : POR·Juanjuan DaiYan Wu
Aug 28, 2021·Frontiers in Cardiovascular Medicine·Selvaraj JayaramanKanagaraj Palaniyandi
Sep 30, 2021·Cellular and Molecular Life Sciences : CMLS·Samiksha KukalRitushree Kukreti
Oct 9, 2021·Chinese Medical Journal·Zi-Nan LuGang Sun

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis
flow cytometry
Assay
transfection
xenograft
immunoprecipitation
ChIP
xenografts
ubiquitination

Software Mentioned

FACSD
ALGGEN
miRbase
Image [UNK]
miRanda
Image J
JASPAR
TargetScan

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.