The Mitochondria-Targeted Antioxidant MitoQ Modulates Mitochondrial Function and Endoplasmic Reticulum Stress in Pancreatic β Cells Exposed to Hyperglycaemia

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Irene Escribano-LopezV M Victor

Abstract

Mitochondria-targeted antioxidants such as mitoquinone (MitoQ) have demonstrated protective effects against oxidative damage in several diseases. The increase in reactive oxygen species (ROS) production during glucose metabolism in β cells can be exacerbated under hyperglycaemic conditions such as type 2 diabetes (T2D), thus contributing to β cell function impairment. In the present work, we aimed to evaluate the effect of MitoQ on insulin secretion, oxidative stress, endoplasmic reticulum (ER) stress and nuclear factor kappa B (NFκB) signalling in a pancreatic β cell line under normoglycaemic (NG, 11.1 mM glucose), hyperglycaemic (HG, 25 mM glucose) and lipidic (palmitic acid (PA), 0.5mM) conditions. We incubated the pancreatic β cell line INS-1E with or without MitoQ (0.5µM) under NG, HG and PA conditions. We then assessed the following parameters: glucose-induced insulin secretion, O₂ consumption (with a Clark-type electrode); mitochondrial function, oxidative stress parameters and calcium levels (by fluorescence microscopy); ER stress markers and NFκB-p65 protein levels (by western blotting). MitoQ increased insulin secretion and prevented the enhancement of ROS production and O₂ consumption and decrease in GSH levels that ...Continue Reading

Citations

Aug 21, 2020·Critical Reviews in Food Science and Nutrition·Jing YangJiajia Song
Aug 3, 2019·European Journal of Medicinal Chemistry·Lucia BiasuttoMario Zoratti
Oct 22, 2019·Journal of Molecular Biology·Leticia P Roma, Jean-Christophe Jonas
Jan 11, 2020·Molecular Metabolism·Matteo BaucknehtCecilia Marini
Feb 7, 2021·Pharmacology Research & Perspectives·Brian D FinkWilliam I Sivitz
Feb 7, 2021·International Journal of Molecular Sciences·Natsuki EguchiHirohito Ichii
Apr 4, 2021·Antioxidants·Štěpánka BenákováLydie Plecitá-Hlavatá

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