The mitochondrial ABC transporter Atm1p functions as a homodimer

FEBS Letters
Maja ChloupkováDavid M Koeller

Abstract

The ATP-binding cassette (ABC) transporters constitute one of the largest families of proteins in evolution. The ATM1 gene of the yeast Saccharomyces cerevisiae encodes an ABC protein, which is localized to the mitochondrial inner membrane. A deletion of ATM1 results in the accumulation of up to a 30-fold excess of mitochondrial iron, loss of mitochondrial cytochromes and abnormalities of cytosolic iron metabolism. In this study, we have evaluated the role of conserved sequence elements in Atm1p in its function and dimerization in vivo. We report that conserved residues in the Walker A and B motifs of the nucleotide binding domain, which are required for ATP binding and hydrolysis, are essential for Atm1p function. In addition, we provide evidence that ATP binding is important for Atm1p dimerization.

References

Jan 1, 1992·Annual Review of Cell Biology·C F Higgins
Jan 1, 1991·Methods in Enzymology·M P Yaffe
Dec 1, 1995·Current Opinion in Genetics & Development·M Dean, R Allikmets
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·N Shani, D Valle
Feb 1, 1997·Nature Genetics·A Decottignies, A Goffeau
Aug 26, 1998·Methods in Enzymology·D Taglicht, S Michaelis
Oct 16, 1999·FEMS Microbiology Letters·P M Jones, A M George
Mar 4, 2000·The Journal of Biological Chemistry·P E LapinskiM Raghavan
Jul 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Erin E Fetsch, Amy L Davidson
Jul 24, 2003·Journal of Molecular Biology·Maja ChloupkováDavid M Koeller
Sep 30, 2003·The Journal of Biological Chemistry·Chun-An Chen, J A Cowan
Mar 6, 2004·The Journal of Biological Chemistry·Junkang XuJian-Ting Zhang
Aug 27, 2005·Mitochondrion·N D SchueckD M Koeller

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Citations

Sep 20, 2011·The Journal of Biological Chemistry·Tibor BedekovicsGrazia Isaya
Feb 8, 2006·Plant Physiology·Do-Young KimYoungsook Lee
Jul 11, 2006·Annual Review of Cell and Developmental Biology·Roland Lill, Ulrich Mühlenhoff
Jan 13, 2006·Annual Review of Pharmacology and Toxicology·P Krishnamurthy, J D Schuetz
Jan 13, 2006·FEBS Letters·Helmut Jungwirth, Karl Kuchler
Jan 31, 2012·Biochemical Pharmacology·Yu Fukuda, John D Schuetz
Jul 28, 2007·Translational Research : the Journal of Laboratory and Clinical Medicine·Michael A Burke, Hossein Ardehali
Mar 3, 2009·Biochimica Et Biophysica Acta·Ariane ZutzRobert Tampé
Apr 28, 2020·Metallomics : Integrated Biometal Science·Stephen A PearsonJ A Cowan
Jan 31, 2014·American Journal of Physiology. Renal Physiology·Min-Hwang ChangMichael F Romero

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