The mitochondrial citrate carrier (CIC) is present and regulates insulin secretion by human male gamete

Endocrinology
Anna Rita CappelloSaveria Aquila

Abstract

The mechanisms through which sperm manage their energy metabolism are poorly understood. The present study provides biochemical and morphological evidence that mitochondrial citrate carrier (CIC) is present in ejaculated human sperm and is restricted to the midpiece. The inhibition of CIC with the specific substrate analog 1,2,3-benzenetricarboxylate resulted in the reduction of cholesterol efflux, protein tyrosine phosphorylation, phospho-AKT, phospho-p60src, hyperactivated motility and acrosome reaction, suggesting a role for this mitochondrial carrier in sperm physiology. Furthermore, inhibition of CIC by 1,2,3-benzenetricarboxylate resulted in a reduction of glucose-stimulated insulin secretion and autocrine insulin secretion by sperm. Remarkably, blocking CIC also reduced glucose-6-phosphate dehydrogenase activity, probably in accordance with its regulation on insulin secretion. Capacitation and glucose metabolism were stimulated by glucose as well as citrate, the specific substrate of CIC, implying a similar action because glucose and citrate both induced insulin secretion by sperm. In the present finding, we discovered a new site of action for CIC in the regulation of metabolism, and it may be assumed that CIC works with...Continue Reading

References

Aug 15, 1978·European Journal of Biochemistry·J Calvin, P K Tubbs
Sep 19, 1990·Biochimica Et Biophysica Acta·F BisacciaF Palmieri
Nov 23, 1989·Biochimica Et Biophysica Acta·F BisacciaF Palmieri
Dec 30, 1974·Biochimica Et Biophysica Acta·A J Meijer, K Van Dam
Apr 24, 1972·European Journal of Biochemistry·M Klingenberg
Jan 1, 1995·Annual Review of Biochemistry·C B Newgard, J D McGarry
Apr 5, 1993·Biochimica Et Biophysica Acta·F BisacciaF Palmieri
Nov 1, 1994·Zygote : the Biology of Gametes and Early Embryos·R Yanagimachi
Jun 25, 1996·Biochemical and Biophysical Research Communications·V ZaraF Palmieri
Jan 1, 1997·Reproduction, Fertility, and Development·A R Jones
Jul 23, 1998·Biology of Reproduction·N L Cross
Feb 26, 1999·The Journal of Biological Chemistry·C A AspinwallR T Kennedy
Sep 8, 2001·Reproduction, Fertility, and Development·A R Jones, D E Connor
Jul 11, 2002·The Journal of Clinical Endocrinology and Metabolism·Saveria AquilaSebastiano Andò
Jul 23, 2003·Human Reproduction·Saveria AquilaSebastiano Andò
Nov 5, 2003·Pflügers Archiv : European journal of physiology·Ferdinando Palmieri
Nov 19, 2003·FEBS Letters·Silvia MarinMarta Cascante
Nov 20, 2004·Endocrinology·Saveria AquilaSebastiano Andò
Jun 10, 2005·The Journal of Clinical Endocrinology and Metabolism·Saveria AquilaSebastiano Andò
Dec 20, 2005·Molecular Reproduction and Development·Antonio MedranoJoan E Rodríguez-Gil
Sep 27, 2006·The Journal of Biological Chemistry·Jamie W JosephChristopher B Newgard
Jul 28, 2007·Human Reproduction·Saveria AquilaSebastiano Andò
Jul 24, 2008·The International Journal of Developmental Biology·Bayard T Storey
Jun 6, 2009·Journal of Cellular Physiology·Saveria AquilaMaurizio Bifulco

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Citations

May 3, 2014·BioMed Research International·Alessandra Ferramosca, Vincenzo Zara
Sep 13, 2014·Nature Reviews. Drug Discovery·Frank MadeoGuido Kroemer
Jan 26, 2013·Biochimica Et Biophysica Acta·M G AlvesP F Oliveira
Jul 16, 2013·Biochimica Et Biophysica Acta·Paola LunettiLoredana Capobianco
Jan 22, 2014·Biological Chemistry·Vito Iacobazzi, Vittoria Infantino
Jan 14, 2017·Systems Biology in Reproductive Medicine·Arvand AsghariNaser Ansari-Pour
Jun 21, 2018·Reproduction : the Official Journal of the Society for the Study of Fertility·R MalivindiS Aquila
May 16, 2014·Advances in Nutrition·Alessandra Ferramosca, Vincenzo Zara
Apr 1, 2020·Animals : an Open Access Journal From MDPI·Blanka Premrov BajukPaola Roncada
Aug 1, 2016·The Journal of Biological Chemistry·Paola LunettiLoredana Capobianco
Aug 20, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Ruyi PengJing Liu

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