The mitochondrial external NADPH dehydrogenase modulates the leaf NADPH/NADP+ ratio in transgenic Nicotiana sylvestris

Plant & Cell Physiology
Yun-Jun LiuAllan G Rasmusson

Abstract

Plant mitochondria contain alternative external NAD(P)H dehydrogenases, which oxidize cytosolic NADH or NADPH and reduce ubiquinone without inherent linkage to proton pumping and ATP production. In potato, St-NDB1 is an external Ca2+-dependent NADPH dehydrogenase. The physiological function of this enzyme was investigated in homozygous Nicotiana sylvestris lines overexpressing St-ndb1 and co-suppressing St-ndb1 and an N. sylvestris ndb1. In leaf mitochondria isolated from the overexpressor lines, higher activity of alternative oxidase (AOX) was detected. However, the AOX induction was substantially weaker than in the complex I-deficient CMSII mutant, previously shown to contain elevated amounts of NAD(P)H dehydrogenases and AOX. An aox1b and an aox2 gene were up-regulated in CMSII, but only aox1b showed a response, albeit smaller, in the transgenic lines, indicating differences in AOX activation between the genotypes. As in CMSII, the increase of AOX in the overexpressing lines was not due to a general oxidative stress. The lines overexpressing St-ndb1 had consistently lowered leaf NADPH/NADP+ ratios in the light and variably decreased levels in darkness, but unchanged NADH/NAD+ ratios. CMSII instead had similar NADPH/NADP+ and...Continue Reading

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Citations

May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Judith ScharteAntje von Schaewen
Jun 25, 2014·Antioxidants & Redox Signaling·Peter Geigenberger, Alisdair R Fernie
Aug 28, 2014·Journal of Biotechnology·Lutz Wobbe, Claire Remacle
Mar 3, 2018·International Journal of Molecular Sciences·Renuka KolliChris Carrie
Mar 20, 2010·Biochemical Society Transactions·Allan G Rasmusson, Sabá V Wallström
Sep 17, 2021·The Plant Journal : for Cell and Molecular Biology·Ian Max MøllerOlivier Van Aken

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