The mitochondrial fission regulator DRP3B does not regulate cell death in plants

Annals of Botany
Keiko YoshinagaMaki Kawai-Yamada

Abstract

Recent reports have described dramatic alterations in mitochondrial morphology during metazoan apoptosis. A dynamin-related protein (DRP) associated with mitochondrial outer membrane fission is known to be involved in the regulation of apoptosis. This study analysed the relationship between mitochondrial fission and regulation of plant cell death. Transgenic plants were generated possessing Arabidopsis DRP3B (K56A), the dominant-negative form of Arabidopsis DRP, mitochondrial-targeted green fluorescent protein and mouse Bax. Arabidopsis plants over-expressing DRP3B (K56A) exhibited long tubular mitochondria. In these plants, mitochondria appeared as a string-of-beads during cell death. This indicates that DRP3B (K56A) prevented mitochondrial fission during plant cell death. However, in contrast to results for mammalian cells and yeast, Bax-induced cell death was not inhibited in DRP3B (K56A)-expressing plant cells. Similarly, hydrogen peroxide-, menadione-, darkness- and salicylic acid-induced cell death was not inhibited by DRP3B (K56A) expression. These results indicate that the systems controlling cell death in animals and plants are not common in terms of mitochondrial fission.

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Citations

Dec 14, 2012·Antioxidants & Redox Signaling·Markus Schwarzländer, Iris Finkemeier
Mar 24, 2017·Plant Science : an International Journal of Experimental Plant Biology·Diego Darío FanelloJuan José Guiamet
Mar 25, 2006·Journal of Experimental Botany·David C Logan

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis