Abstract
Wolbachia, a genus of endosymbiotic bacteria of filarial worms, represent novel targets for anti-filarial therapy. The efficacy of compounds against Wolbachia has been evaluated using antiserum raised against the 60 kDa heat shock protein (HSP60) which binds specifically to this protein in both Wolbachia and mitochondria. It has been shown that Wolbachia stains (using such specific probes) stronger than the mitochondria in untreated Onchocerca volvulus, whereas after the depletion of Wolbachia (with drugs) staining of the mitochondria is increased. Herein, immunogold electron microscopy showed that specific anti-HSP60 serum specifically labelled Wolbachia and filarial mitochondria, and that both have distinct localization patterns, thus allowing them to be differentiated. Immunohistochemistry of O. volvulus showed that HSP60 staining is increased in the mitochondria after Wolbachia depletion in the hypodermis, epithelia, muscles, oocytes, embryos, and developing spermatozoa. This could have been the result of the antiserum preferentially binding to the Wolbachia when they are present or due to increased expression of the protein in the absence of the bacteria. To address this, mRNA levels of filarial hsp60 in O. volvulus were m...Continue Reading
References
Jul 1, 1977·The American Journal of Tropical Medicine and Hygiene·W J Kozek, H F Marroquin
Jan 1, 1995·Parasitology Research·A Plenge-BönigD W Büttner
Jan 12, 1999·The Journal of Clinical Investigation·A HoeraufB Fleischer
Jan 28, 1999·Proceedings. Biological Sciences·C BandiM L Blaxter
Oct 8, 1999·Parasitology Today·M J Taylor, A Hoerauf
Apr 26, 2000·Molecular and Biochemical Parasitology·Y WuA E Bianco
Aug 29, 2002·The EMBO Journal·Quan ZhaoNicholas J Hoogenraad
Dec 3, 2002·Parasitology·B O L DukeM Boussinesq
Mar 13, 2003·Parasitology Research·L H KramerM Casiraghi
Apr 23, 2003·Microbes and Infection·Achim HoeraufDietrich W Büttner
May 23, 2003·The Journal of Parasitology·S C BarrJ S Chandler
Jun 13, 2003·Filaria Journal·Dietrich W BüttnerPeter Fischer
Oct 29, 2003·Trends in Parasitology·David H MolyneuxMark J Taylor
Jun 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Norbert W BrattigAchim Hoerauf
Jul 29, 2004·Journal of Cell Science·Takunari YonedaDavid Ron
Mar 23, 2005·PLoS Biology·Jeremy FosterBarton Slatko
Apr 21, 2005·PLoS Medicine·Kenneth Pfarr, Achim Hoerauf
Jun 21, 2005·Lancet·Mark J TaylorAchim Hoerauf
Sep 20, 2005·The Journal of Infectious Diseases·Jeffrey GilbertAlexander J Trees
Oct 19, 2005·Advances in Parasitology·Mark J TaylorAchim Hoerauf
Apr 7, 2006·Expert Review of Anti-infective Therapy·Achim Hoerauf
Apr 18, 2006·International Journal of Medical Microbiology : IJMM·Ulrike HeiderKenneth M Pfarr
Sep 28, 2006·PLoS Medicine·María-Gloria BasáñezMichel Boussinesq
Apr 7, 2007·Cornea·Steven E Wilson, R Doyle Stulting
Citations
May 20, 2009·Parasitology Research·Simone KortenNorbert Brattig
Jul 25, 2009·Parasitology Research·Klaus D Erttmann, Dietrich W Büttner
Sep 29, 2009·Parasitology Research·Sabine SpechtDietrich W Büttner
Sep 17, 2009·Parasitology Research·Sabine SpechtDietrich W Büttner
Mar 5, 2010·Parasitology·N W BrattigD W Büttner
Oct 7, 2009·PLoS Neglected Tropical Diseases·Elodie GhedinSara Lustigman
Oct 15, 2010·Genetics·Meghan E ChafeeSeth R Bordenstein
Apr 7, 2010·International Journal for Parasitology·Uta StrübingKenneth M Pfarr
Dec 6, 2012·Cellular Microbiology·Mark J TaylorLouise Ford
Aug 15, 2012·Experimental Parasitology·R C MisraP K Murthy
Nov 21, 2018·Behavior Genetics·Daniela I SchneiderWolfgang J Miller