The mitochondrial inner membrane protein LETM1 modulates cristae organization through its LETM domain.

Communications Biology
Seiko NakamuraToshihiko Oka

Abstract

LETM1 is a mitochondrial inner membrane protein that is required for maintaining the mitochondrial morphology and cristae structures, and regulates mitochondrial ion homeostasis. Here we report a role of LETM1 in the organization of cristae structures. We identified four amino acid residues of human LETM1 that are crucial for complementation of the growth deficiency caused by gene deletion of a yeast LETM1 orthologue. Substituting amino acid residues with alanine disrupts the correct assembly of a protein complex containing LETM1 and prevents changes in the mitochondrial morphology induced by exogenous LETM1 expression. Moreover, the LETM1 protein changes the shapes of the membranes of in vitro-reconstituted proteoliposomes, leading to the formation of invaginated membrane structures on artificial liposomes. LETM1 mutant proteins with alanine substitutions fail to facilitate the formation of invaginated membrane structures, suggesting that LETM1 plays a fundamental role in the organization of mitochondrial membrane morphology.

References

Feb 2, 2002·The EMBO Journal·Patrick PaumardJean Velours
Mar 22, 2002·Molecular Biology of the Cell·Kai Stefan DimmerBenedikt Westermann
Feb 19, 2003·Biochemical and Biophysical Research Communications·Naotada IshiharaKatsuyoshi Mihara
Sep 10, 2004·Molecular Biology of the Cell·Yang-ja LeeRichard J Youle
May 19, 2005·The Journal of Biological Chemistry·Hsiuchen ChenDavid C Chan
Feb 16, 2006·The Journal of Cell Biology·Ann E FrazierPeter Rehling
Feb 16, 2007·The Journal of Biological Chemistry·Naoko TaguchiKatsuyoshi Mihara
Apr 28, 2007·Cell Death and Differentiation·L Pellegrini, L Scorrano
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Gerrit van MeerGerald W Feigenson
Mar 8, 2008·The EMBO Journal·Mike StraussWerner Kühlbrandt
Jun 17, 2009·The Journal of Cell Biology·Regina RablAndreas S Reichert
Dec 23, 2009·Human Molecular Genetics·Angus G McQuibbanKarin Nowikovsky
Jun 6, 2012·The International Journal of Biochemistry & Cell Biology·Johan HabersetzerPatrick Paumard
Aug 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Karen M DaviesWerner Kühlbrandt
Feb 26, 2013·Biochimica Et Biophysica Acta·Hidenori OteraKatsuyoshi Mihara
May 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Dawei JiangDavid E Clapham
Jul 31, 2013·The Journal of Biological Chemistry·Hassan HashimiJulius Lukeš
Dec 18, 2013·The Journal of General Physiology·Ming-Feng TsaiChristopher Miller
Aug 5, 2015·American Journal of Medical Genetics. Part C, Seminars in Medical Genetics·Agatino BattagliaSarah T South
Jun 2, 2016·Cell and Tissue Research·Vera Kozjak-Pavlovic
May 21, 2017·Biochimica Et Biophysica Acta. Molecular Cell Research·Florian WollweberMartin van der Laan
Dec 6, 2017·Frontiers in Physiology·Shane AustinKarin Nowikovsky
Jun 1, 2018·Methods in Molecular Biology·Jan SuskiMariusz R Wieckowski

❮ Previous
Next ❯

Citations

Aug 8, 2020·Stem Cells Translational Medicine·Sara Mirali, Aaron D Schimmer
May 28, 2020·International Journal of Molecular Sciences·Megan NoblePeter B Stathopulos
Feb 13, 2021·Protein Science : a Publication of the Protein Society·Qi-Tong LinPeter B Stathopulos
Feb 23, 2021·Frontiers in Cell and Developmental Biology·Corina T Madreiter-SokolowskiWolfgang F Graier
Mar 9, 2021·Biochimica Et Biophysica Acta. Molecular Cell Research·Maria Calvo-RodriguezBrian J Bacskai
Apr 4, 2021·International Journal of Molecular Sciences·Hyon Dok SongYu Wang
Apr 6, 2021·Frontiers in Physiology·Gayathri K NatarajanWai-Meng Kwok
Jun 5, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Guilherme Rodrigo Rm Dos SantosRoberto Docampo
Aug 25, 2021·The International Journal of Biochemistry & Cell Biology·R Viswambari DeviMukesh Doble
Oct 27, 2021·Physiological Reviews·Joanne F Garbincius, John W Elrod

❮ Previous
Next ❯

Methods Mentioned

BETA
confocal microscopy
electron microscopy
electrophoresis
affinity column chromatography
GTPase
transfection
PCR
cross-linking

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.