PMID: 3384093Jun 20, 1988Paper

The mitochondrial probe rhodamine 123 inhibits in isolated hepatocytes the degradation of short-lived proteins

FEBS Letters
J L VargasS Grisolía

Abstract

The fluorescent dye rhodamine 123 (R123) decreases the intracellular ATP levels and also inhibits the degradation of short-lived proteins in isolated hepatocytes. This inhibition affects lysosomal and, to some extent, non-lysosomal mechanisms. The degradation of short-lived proteins decreases more when ATP levels are less than 40% of those in control cells, in contrast to the reported linear correlation between ATP levels and degradation of long-lived proteins. R123 provides a powerful probe for clarifying the proteolytic mechanisms involved in degradation of short-lived proteins and the ATP requirements in protein degradation. Indeed, as illustrated, the results suggest different mechanisms for the degradation of short- and long-lived proteins. Moreover, they provide a warning for the clinical use of this reagent.

References

Nov 1, 1975·Analytical Biochemistry·G A KimmichJ S Brand
Jan 20, 1977·Biochimica Et Biophysica Acta·J Mørland, A Bessesen
Mar 31, 1986·FEBS Letters·R J Mayer, F Doherty
Sep 15, 1987·Biochemical and Biophysical Research Communications·J L VargasS Grisolia
Apr 1, 1987·European Journal of Biochemistry·P J PlompP O Seglen
Feb 14, 1984·Biochemical and Biophysical Research Communications·J S Modica-NapolitanoJ R Aprille
Jan 1, 1982·Annual Review of Biochemistry·A Hershko, A Ciechanover
Jan 1, 1983·Methods in Enzymology·P O Seglen
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L V JohnsonL B Chen
Sep 21, 1982·Journal of Theoretical Biology·D N WheatleyJ Hernández-Yago

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